Intro I (Reviewer) (RVSP) Flashcards

1
Q

Essential part of a microtome; for actual cutting of tissue sections

A

Knife carrier & knife

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2
Q

Essential part of a microtome; where the tissue is held in position

A

Block holder

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3
Q

Essential part of a microtome; used to line up the tissue block in proper position with the knife; used to adjust the thickness of the tissue for successive sections

A

Ratchet feed wheel
Pawl
Adjustment screws

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4
Q

Type of microtome; simplest microtome

A

Rocking Microtome

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5
Q

Inventor of the rocking microtome

A

Caldwell Trefall

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6
Q

Inventor of the Rotary Microtome

A

Minot

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7
Q

Inventor of the Freezing Microtome

A

Queckett

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8
Q

Type of microtome; capable of cutting sections for electron microscopy

A

Ultra-thin Microtome

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9
Q

Thickness of sections cut by ultra-thin microtomes

A

0.5 μ

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10
Q

Type of microtome recommended for cutting extremely hard blocks

A

Sliding/Base Sledge Microtome

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11
Q

Inventor of the Sliding/Base Sledge Microtome

A

Adams

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12
Q

The histology technician’s tool of his profession

A

Microtome Knife

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13
Q

Types of microtomes that use biconcave microtome knives

A

Rocking microtome
Rotary microtome
Carbon dioxide-freezing microtome

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14
Q

The plane-concave microtome knife with lesser concavity is used for

A

Celloidin sections

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15
Q

The plane-concave microtome knife with more concavity is used for

A

Paraffin sections on the sledge, rotary or rocking microtome

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16
Q

The plane-wedge microtome knife is routinely used for

A

Frozen sections and extremely hard specimens embedded in paraffin wax; also for paraffin blocks cut on the rotary microtome

17
Q

3 types of microtome knives

A

Biconcave
Plane-concave
Plane-wedge

18
Q

2 chemicals that can be used to clean microtome knives

A

Xylene

Toluene

19
Q

Temperature of Cryostat

A

-20°C

20
Q

Machine on which sections are cut

A

Microtome