Intro

Question 1

What is CLIA and when was it passed

Answer 1

1988 clinical lab improvement amendments, helps to ensure, accuracy, reliability, and timliness of lab tests (congressmans wife pap smear)

Question 2

CLIA doesn’t apply to

Answer 2

home tests

Question 3

provider must obtain a _________ for provider performed microscopy procedures (PPMP)

Answer 3

CLIA certificate

Question 4

rainbow tubes include ___, ____, and _____ colored tops useful for _____ testing

Answer 4

red, blue, and purple; blood testing

Question 5

Lavender/purple top tubes contain ________ as a preservative and anticoagulant. It works by binding up ____ (chelation).

Answer 5

K+ EDTA; binds up calcium

Question 6

Lavender/purple tops are useful for

Answer 6

hematology as the preserve the cell’s morphology ; makes plasma

Question 7

Red tops (or yellow SST) contain no ________, produces _________,

Answer 7

no anticoagulant, produce serum

Question 8

Red tops/ yellow serum seperating tubes are useful why?

Answer 8

RBCs metabolize glucose, so it’s good to have a barrier between RBCs and serum

Question 9

shaking a tube vigorously can result in

Answer 9

hemolysis

Question 10

Serum vs Plasma

Answer 10

serum is not circulating, it has no coag factors (no fibrinogen); liquid portion part of blood after coagulation.
Plasma contains fibrinogen, liquid cell free portion treated with fibrinogen

Question 11

Why shouldn’t you refridgerate a CBC

Answer 11

platelets don’t work when cold

Question 12

Light Blue tops contain _________ that is a ________chelator. With these tubes it’s critical to…

Answer 12

Na+ citrate; that is a calcium chelator. crutial to main the 1:10 ratio of blood to anticoag in the tube (DON’T MIX TUBES)

Question 13

What are blue tops useful for?

Answer 13

routine coag studies, INR, PTT, fibrinogen, PLT activity assays

Question 14

START AT GREEN TOP

Answer 14

yup