Intro Flashcards
Deals with the study of abnormal tissue
histopathology
study of normal tissues aka microscopic anatomy
Histology
art and science performed by the medtech to produce good quality tissue sections
Histopathologic techniques
3 spx processed in the lab
Cytology, Biopsy spx and autopsy spx
spx from alive person
biopsy spx
remove only part of mast or organ
incisional
removal of entire mast or organ
excisional
use of needle and syringe to collect tissues
FNAB
aka necropsy
autopsy
from a diseased person
autopsy spx
To determine the cause of death: autopsy
to detect cancer cells:
biopsy
performed by surgeon: biopsy
performed by pathologist:
autopsy
cuts use for infants
straight cut
cuts use for adults
y shaped cut
examination of whole body from head to toe
complete autopsy
examination of a region of the body
Partial autopsy
after opening the body the next step is?
Evisceration
Autopsy techniques where organs are removed one by one
R. Virchow
father of Cytology
George Papanicolau
father of modern pathology
R. Virchow
In situ technique (in its original place)
C. Rokitansky
autopsy technique through en-bloc or per system
A.Ghon
en-masse: removal of all the organs at the same time
M. Letulle
screening test for cervical cancer
PAPS smear
lung spx thickness and size
size: 3 x 2 cm
thickness! 1-2 cm
Mortal sin in Histopath section
Mislabelling
Tissue is placed in a watch glass with NSS
Teasing/ Dissociation
small tissues placed on a slide and compressed with another slide
Squash preparation/ crushing
type of smear preparation that applied on a slide in a zigzag manner
streaking
type of smear prep that do circular motion
spreading
uses 2 slide under smear preparation
pull-apart
type pf smear preparation that preserve intercellular relationship
spreading
Method that is recommended in dealing with cells
smear preparation
allow slide to come in contact with freshly cut tissues
abraded cytology/ impression smear/ touch preparation
TAT in frozen section
5-15 minutes
apparatus used in frozen section
Freezing microtome and Cryostat/ cold microtome
1st and most important step
Numbering/ accessioning
Size of spx required
3 x 2 cm
thickness of spx required
3-5 mm
Tissue cassette size
2.5 x 4 cm
depth: 5mm
manner on how the disease had developed
Pathogenesis
refers to incomplete or defective development of a tissue or organ
Aplasia
usually happen in paired structures like kidneys and gonads
aplasia
the affected organ shows no resemblance to the normal adult structure
aplasia
refers to complete non appearance of an organ / absence of an organ
agenesia
refers to failure of an organ to reach its normal mature adult size
Hypoplasia
failure to from an opening
atresia
imperforate anus
atresia
microtia
atresia
type of injury where affected cells can get back to its normal state
Reversible injury
type of injury where affected cells can no longer back to its normal state
irreversible injury
considered the point of no return
Irreversible injury
Patterns of cell death
Apoptosis and Necrosis
