Intro Flashcards
Identify each type of stain and what type of structure it can be used to stain. Also the type of microscope that has produced an image.
Hematoxylin
Blue or Purple Base-like stain (binds to basophillic structures) (basophillic structures are acidic) structures: nucleotides (DNA, RNA and RER) also glucosaminoglycans (GAGs)
Eosin
Red, Pink or Orange Acid-like stain (binds to acidophillic structures)
(acidophillic structures are basic)
structures: mitochondria, proteins (collagen etc.)
Types of microscopy
Light, transmission electron microscopy (as well as freeze fracture, and scanning electron microscopy
Scanning electron microscopy (SEM)
shows external (or surface) structure
transmission electron microscopy (TEM)
Shows internal structure
TEM freeze fracture
splits cell membranes
What does periodic-acid schiff (PAS) stain for?
stains carbohydrates magenta (e.g. glycoproteins)
What does trichrome stain for?
stains collagen blue or green
What does silver stain for?
Stains reticular fibers black
What does orcein stain for?
Stains elastic fibers reddish-brown or black
What does sudan red stain for?
Stains lipids RED
What does osmic acid stain for?
Stains lipids BLACK
What is the diameter of a RBC?
8 microns
artifacts
tissue shrinkage and lost molecules
