Instrumentation and sampling

Question 1

What occurs in the sampling process

Answer 1

A few milligrams of sample is mixed with a generally organic solvent so that a homogeneous solution is formed and placed into a glass tube

Question 2

Explain what the instrumentation does to analyse the sample

Answer 2

1- Places the sample in a magnetic field (B0) to generate two accessible energy levels for the nuclei.
2- Irradiate the sample with an electromagnetic radiation of varying wavelength (l)
3- Detect the wavelengths that correspond to energy transitions between the accessible energy levels.
4- Processing of the detected signals and print out the result/save file.

Question 3

Why is the solvent used to prepare the sample deuteriated

Answer 3

This is because if not the H would appear on the analysis interrupting the results

Question 4

What else does the chemical shift depend on

Answer 4

The solvent used