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Clinical Chemistry Lab > Instrument Used in CC1 > Flashcards

Instrument Used in CC1 Flashcards

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1
Q

Required for preparation of any primary standard. Single pan enclosed by sliding transparent doors.

A

Analytical balances

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2
Q

Single pan balances. Uses an electromagnetic force to counterbalance the weighed sample’s sample.

A

Electronic balances

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3
Q

Consists of a head or rotor, carriers, or shields that are attached to the vertical shaft of a motor or air compressor and enclosed in a metal covering

A

Clinical centrifuge

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4
Q

process in which centrifugal force is used to separate solid matter from a liquid suspension; also separate two liquid phases of different densities.

A

Centrifugation

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5
Q

RCF in grams

A

1.118 x 10-5 x r x (rpm)2 ; or use nomogram

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6
Q

RPM

A

tachometer or strobe light

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7
Q

tubes placed in the cups of the rotor assume a horizontal plane when the rotor is in motion and vertical position when at rest.

A

Horizontal-head or swinging-bucket centrifuge

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8
Q

During centrifugation, particles travel in a constant manner along the tube while the tube is at right angle to the shaft of the centrifuge. The surface of the sediment is flat.

A

Horizontal-head or swinging-bucket centrifuge

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9
Q

Tubes are held at a fixed angle from 25-40 degrees to the vertical axis of rotation.

A

Angle-head or fixed angle centrifuge

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10
Q

Particles are driven outside and bottom of the tube and the surface of the sediment packs against the side and bottom of the tube and the surface of the sediment is parallel to the shaft of the centrifuge.

A

Angle-head or fixed angle centrifuge

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11
Q

High-speed centrifuge; mainly fixed angle rotors requires a refrigerated chamber.

A

Ultracentrifuge

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12
Q

circulating or noncirculating. maintenance: Type II (or Type I) water

A

Water bath

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13
Q

List of Transfer Pipets

A
  1. Volumetric or Transfer pipet
  2. Ostwald-Folin pipet
  3. Pasteur pipets
  4. Automatic (macro or micropipets)
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14
Q

 used to transfer aqueous solutions & non-viscous samples
 self-draining

A

Volumetric or Transfer pipet

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15
Q

 has the greatest degree of accuracy and precision
 read at the lower meniscus
 with bulb at the center

A

Volumetric or Transfer pipet

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16
Q

 used for biologic fluids having viscosity greater than water
 blowout pipets

A

Ostwald-Folin pipet

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17
Q

 read on the upper meniscus
 bulb is closer to the delivery tip

A

Ostwald-Folin pipet

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18
Q

no calibration mark; used to transfer solutions or biologic fluids without consideration of a specific volume

A

Pasteur pipets

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19
Q

capable of dispensing several used to deliver a predetermined volume of liquid.

A

Graduated or Measuring Pipets

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20
Q

 calibrated between two marks; deliver between their calibration marks
 Tip should NOT touch the receiving vessel
 self-draining; with smaller orifice

A

Mohr pipet

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21
Q

 graduated down to the tip
 blow-out pipet; with larger orifice

A

Serological pipet

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22
Q

exact volume is calibrated to fill the volume between two calibration points on the pipet

A

“Between Two Marks”

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23
Q

 Holds the particular volume but does not dispense the exact volume
 Requires rinsing
 calibrated with mercury
 usually a micropipette - Volumes are expressed in microliter

A

“To-Contain” (TC)

24
Q

 Delivers the exact volume indicated; Calibrated for the volume delivered
 fluid is allowed to flow freely with the pipet tip touching the inner wall of receiving vessel

A

“To-Deliver” (TD)

25
Q

 Safer, less time consuming, precise & convenient
 mechanism draws up and dispenses the liquid

A

Automatic macropipets or micropipets

26
Q

relies on a piston for suction creation to draw the sample into a
disposable tip.

A

Air-displacement

27
Q

operates by moving the piston in the tip or barrel.
 Sample enters directly upon contact without air interference.
 NO need to replace delivery tip.

A

Positive displacement

28
Q

obtain the liquid from a common reservoir and dispense it
repeatedly; combines sampling & dispensing functions

A

Dilutor/Dispenser pipets

29
Q

delivering and weighing a solution of known specific
gravity.

A

Gravimetric Method

30
Q

the molar extinction coefficient of a compound, such as potassium dichromate is obtained.

A

Spectrophotometric Method -

31
Q

 has a round, lower portion with with a flat bottom and a long, thin neck with an etched calibrated line

A

Volumetric Flask

32
Q

 For preparation of standards for quantitative procedures.
 Class A , TC (To-Contain)

A

Volumetric Flask

33
Q

cylindrical shaped calibrated plastic or glass containers.

A

Centrifuge Tube

34
Q

 13mm x 100mm
 Constant light path: 1 cm distance

A

Cuvets

35
Q

used to contain acidic solutions

A

SOFT

36
Q

“Borosilicate” for alkaline solutions

A

HARD

37
Q

TYPES as to COMPOSITION:

A

Soft, Hard

38
Q

TYPES as to WAVELENGTH SETTINGS

A

Glass, Quartz

39
Q

TYPES as to SHAPE

A

Round, Square and Rectangle

40
Q
  1. GLASS: for ____________ settings (____ – 700 nm or ____ – 700 nm)
  2. QUARTZ: for ___________________ settings (UVR: below ___ nm)
A
  1. GLASS: for wide-range settings (300 – 700 nm or 380 – 700 nm)
  2. QUARTZ: for short wavelength settings (UVR: below 340 nm)
41
Q
  1. ROUND – ______
  2. SQUARE & RECTANGLE – __________________
    PROPER HANDLING: No ___________, no _____________, and no ___________________
A
  1. ROUND – glass
  2. SQUARE & RECTANGLE – quartz/plastic
    PROPER HANDLING: No scratches, no detergents, and no sterilization
42
Q

For transfer of smaller volumes (<500ul) in blood gas analyis or in separationtechniques (chromatography, electrophoresis).

A

Syringes

43
Q

SPECIAL GLASSWARES:

A
  1. Colored and Opal Glasses
  2. Coated Glass
  3. Optical Glass
  4. Glass Ceramics
  5. Radiation-Absorbing Glass
44
Q

 has metallic oxides; used for filters and light bulbs

A

Colored and Opal Glasses

45
Q

 has a thin, metallic oxide permanently fire-bonded to its surface; canconductelectricity
 has electronic applications as a heat shield to protect against infraredlight andasan electrostatic shield to carry off charges

A

Coated Glass

46
Q

 made of soda lime, lead and borosilicate
 has a high optical activity; prisms, lenses and optical mirrors

A

Optical Glass

47
Q

 with high thermal resistance, chemical stability and corrosion resistance
 for hot plates, table tops and heat exchangers

A

Glass Ceramics

48
Q

made of soda lime and lead

A

Radiation-Absorbing Glass

49
Q

 Clear and rigid; not autoclavable
 Used for disposable wares

A

Polystyrene (PS)

50
Q

 Not recommended for use with acids, aldehydes, ketones, ethers, hydrocarbonsor essential oils.
 Alcohols and bases can be used but not to be stored longer than 24 hours.

A

Polystyrene (PS)

51
Q

 Chemically resistant to most substances except for aldehydes, amines, ethers, hydrocarbons and essential oils
 ____________________________: translucent and flexible; not autoclavable

A

Polyethylene
Conventional Polyethylene (CPE)

52
Q

 Has the same chemical resistant as polyethylene
 Translucent and rigid; autoclavable; Used for screw-cap closures

A

Polypropylene (PP)

53
Q

 Resin that has excellent chemical resistance to almost all chemicals in the lab.
 Clear, translucent and flexible; autoclavable
 Used for stopcocks, wash bottles and tubings

A

Teflon

54
Q

Translucent and flexible; autoclavable; used for tubings

A

Tygon

55
Q

 Very susceptible to damage by most chemicals. Resistant to water, aqueous salts
and inorganic acids for a long period.  Very clear and rigid; autoclavable
 Used for __________, _________________

A

Polycarbonate (PC)
carboys, test tube racks

Clinical Chemistry Lab (2 decks)

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