Inflammation Flashcards
Define Inflammation
Defensive process that a living body initiates against infection and damaged tissue.
Inflammation recruits cells and molecules from circulation to where they are needed to eliminate offending agents.
An “itis” suffix is put for inflammatory diseases
List the factors that cause inflammation.
Physical agents-temperature
Chemical agents- drugs
Biological agents-Bacteria
Immune reactions- abnormal inflammatory response is chronic inflammation
What are the 5 pillars of inflammation?
Name 4 common side effects of inflammation
Suppurative or purulent inflammation, abscess. Pus consists of neutrophils, dead cells, tissue fluid
Ulcers
Serous inflammation (blisters)
Fibrinous inflammation: fibrin leaks into the tissue from blood vessels causing scaring
What is the difference between chronic and acute inflammation?
Acute inflammation: a rapid response to deliver leucocytes and plasma proteins to infection sites or tissue injury, short duration. E.g. acute appendicitis/ bronchitis, abscess.
Chronic inflammation: prolonged inflammation (weeks/months) where inflammation, tissue injury and attempts at repair coexist. Eg tb, autoimmune disease. Macrophphage, Lymphocytes, Plasma cells involved.
What are characteristics of acute inflammation?
Innate immune response- occurs in mins/hours, lasts for hours/days
Main cell: neutrophil
Mostly local, can be systemic (e.g. fever, neutrophilia)
Exudation of fluid and plasma proteins (edema) + emigration of leukocytes
Obvious clinical signs= 5 pillars
What is the purpose of acute inflammation?
Recognition of injury
Recruitment of leukocytes
Removal of causative agent
Regulation (closure of inflammatory response)
Repair of affected tissue
Describe termination of acute inflammation
V important, controls response and prevents it excessively damaging host tissues
Occurs when causative agent is removed
Mediators broken down and dissipated
Leukocytes have short life span in tissues so die
Anti-inflammatory mechanisms activated.
What are the 4 outcomes of acute inflammation?
Complete resolution: causative agent removed early, limited tissue damage, cells at site able to regenerate
Abscess: purulent inflammation caused by certain bacteria. Can become chronic if not reabsorbed/ drained
Repair by scarring/fibrosis: Greater tissue destruction, native tissue cannot regenerate (eg m.infarction). Depends on tissue regeneration ability (labile> stable >permanent tissues)
Progression to chronic inflammation: Causative agent could not be removed
What are the main events of acute inflammation?
Histamine produced by mast cells attract more mast cells
Vasodilation & stasis widen internal diameter of blood
vessels. Dilated blood vessels but same HR means blood volume ⇡ and slows down. This ensures neutrophil/monocytes migrate to infection site in time.
Endothelial cells contract, ⇡ vascular permeability. Fluid and cells leak out blood vessel walls
Endothelial cells have adhesion molecules for leukocytes to bind by margination. Leukocytes roll along blood vessel walls. Activated integrins on leukocytes binds them tighter to adhesion molecules.
Leukocytes transmigrate into surrounding tissues by chemotaxis, meaning they move to the area w the highest conc of distress molecules. They’re activated. Phagocytosis starts.
The cells that are involved in acute inflammation are the leukocytes of the innate immune response. How do neutrophils behave?
Neutrophils phagocytose pathogens
- Release granules to counteract histamine
- Release chemokines to attract mote leukocytes
- Generate reactive oxygen/nitrogen species to kill
- Form Extracellular Traps (NETs) that trap pathogens
- Short lived, becomes pus
The cells that are involved in acute inflammation are the leukocytes of the innate immune response. How do macrophages and others behave?
Macrophage
- Differentiate from monocyte
- Do phagocytosis
- Release factors that promote tissue repair (TGF-B)
Eosinophils- combat parasites and allergies
Lymphocytes- for viral infections (target cell mediated)
Give mediators and their functions
C5a: -releases oxygen species (for chemical warfare)
- Attracts neutrophils and monocytes
- Increases vascular permeability
- Mast cells release histamine and TNF- alpha
Histamine: vasodilation, contracts endothelial cells
TNF- alpha
- Released by mast calls and macrophages
- causes endothelial adhesion for leukocytes
Others: contribute to the 5 signs of inflammation
What is the main causes of inflammation?
o Persistent infections
o Persistent exposure to agent
o Immune mediated (auto immune disease/ allergies)
What are the mediators of chronic inflammation?
- All the acute mediators
- Chemokines IL17 and IL12
- INF-γ: Produced by T cells and NK cells. It activates macrophages and increases microbicidal activity