Infection And Response Flashcards

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1
Q

Define the Term Pathogen

A

A microorganism that causes an infectious disease.

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2
Q

Explain how Bacteria Make us Feel Ill

A

-Bacterial cells are prokaryotes- single celled organisms. Bacterium cells are very small (1/100th the size of human body cells).

-Bacteria multiply extremely rapidly. Bacteria do not have to enter a cell to multiply so bacteria can easily multiply in our bloodstream and reach very large numbers very quickly.

-Many bacteria release a toxin. Toxins are chemicals which damage cells and tissues and make us feel ill.

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3
Q

Explain how Bacteria Make us Feel Ill

A

-Viruses are not cells and are about 1/100th the size of a bacterium. Unlike bacteria, viruses can only reproduce if they enter a host cell.

-Once inside a host cell, the virus uses the cells’ machinery (e.g. essential molecules) to replicate themselves many times.

-This will continue until the cell bursts open releasing the new copies of the virus. This cell damage is what causes people to feel unwell.

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4
Q

Explain how Protists Make us Feel Ill

A

-Protists are single celled eukaryotes.

-Some protists are parasites. This means they live on or inside other organisms and can cause them damage.

-They are transferred to the other organism by a vector, which doesn’t get the disease itself.

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5
Q

Explain how Fungi Make us Feel Ill

A

-Some fungi are single celled. Others have a body which is made up of a hyphae (thread like structure)

-These hyphae can grow and penetrate human skin and the surface of plants, causing disease.

-The hyphae can produce spores, which can be spread to other plants and animals.

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6
Q

Describe how to Prevent the Spread of Disease

A

Water Borne Diseases (e.g. cholera) - UV treatment, iodine tablets, sterilising the water

Air Borne Diseases (e.g. influenza) - wearing masks, keeping distance, coughing into a tissue

Diseases Spread by Direct Contact (e.g. athlete’s foot) - wash hands regularly, wipe down surfaces, avoid close contact

Diseases Spread Through Bodily Fluids (e.g. HIV) - barrier contraception, don’t use the same needles as others

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7
Q

Describe how to Reduce the Spread of Highly Infectious Diseases such as Ebola

A

-Ebola is a very highly infectious disease. It is very easily spread, unless very strict precautions are taken (for example wearing a protective suit).

-The best way to prevent the spread of diseases like this
is to isolate the patients and not allow anyone to come into contact (apart from medical staff who take precautions).

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8
Q

Describe how to Reduce the Spread of Pathogens for Less Infectious Diseases such as Measles

A

-One of the most effective ways to prevent the spread of less infectious diseases (e.g. Measles) is to vaccinate people against them.

-Because they cannot catch the disease themselves, they cannot spread it to other people.

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9
Q

Describe the Symptoms of Measles

A

-Measles is a viral disease. It can damage the breathing system and the brain.

-The first symptom of measles is a fever (high temperature). The second symptom (after three days) is a red skin rash.

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10
Q

Describe how Measles is Spread

A

-Measles is spread from person to person in droplets.

-When an infected person coughs or sneezes, tiny droplets of water are released, containing the measles virus.

-When these droplets are then inhaled by another person, the virus is passed on.

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11
Q

Describe the Prevention of Measles

A

-Measles is not very common (in the UK) as children are vaccinated against measles when they are very young.

-Neither measles nor HIV can be treated using antibiotics. This is because viruses are not killed by antibiotics

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12
Q

Describe the Symptoms of HIV

A

-HIV is a viral disease. After the initial infection with HIV, someone will experience a flu-like illness lasting one to two weeks.

-The virus will then attack cells of the immune system which becomes severely damaged.

-The patient may then catch an infectious disease e.g. TB or develop cancer. Late stage HIV / AIDS can be fatal.

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13
Q

Describe how HIV is Spread

A

-HIV is transmitted by body fluids.

-It can be transmitted through unprotected sexual intercourse (ie not using a condom) or by intravenous drug users sharing needles (which transmits infected blood).

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14
Q

Describe the Treatment of HIV

A

-Antiretroviral drugs can be taken to control HIV. These stop the virus from multiplying.

-This prevents the patient’s immune system from being damaged by the virus. The patient must take these drugs for the rest of their life.

-Neither measles nor HIV can be treated using antibiotics. This is because viruses are not killed by antibiotics

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15
Q

Describe the Symptoms of Salmonella

A

-Salmonella food poisoning is caused by bacteria.

-The symptoms of salmonella food poisoning are fever, abdominal cramps, vomiting and diarrhoea.

-When salmonella bacteria enter the human body, they release toxins. Toxins are chemicals which can damage cells.

-These toxins cause the symptoms of salmonella food poisoning.

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16
Q

Describe how Salmonella is Spread

A

-A person can become infected by ingesting food which contains salmonella bacteria (e.g. raw chicken).

-This can happen for example if raw chicken is prepared on a chopping board and then the same chopping board is not
cleaned before it is used to prepare other foods e.g. a salad.

-Any salmonella bacteria in the chicken will be killed when it is cooked.

-However, the salmonella bacteria which have contaminated the salad will not be killed and can cause salmonella food poisoning

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17
Q

Describe the Prevention of Salmonella

A

-To prevent salmonella, wash hands and keep uncooked meat away from other foods.

-Salmonella food poisoning is uncommon in the UK as all chickens are vaccinated against the bacteria which cause salmonella.

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18
Q

Describe the Symptoms of Gonorrhoea

A

-Gonorrhoea a sexually transmitted disease (STD) caused by a bacterium.

-It is passed on by sexual contact e.g. having unprotected sex caused by bacterium.

-The symptoms of gonorrhoea are a yellow/green discharge from the penis or vagina.

-Gonorrhoea can also lead to pain when urinating.

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19
Q

Describe the Treatment of Gonorrhoea

A

-Gonorrhoea is treated using antibiotics. However, it is no longer treated with the antibiotic penicillin.

-This is because the bacteria have become resistant to penicillin.

-This means is that the bacteria have changed so they are no longer killed by penicillin.

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20
Q

Describe the Prevention of Gonorrhoea

A

-One way of preventing the spread of gonorrhoea is to use a condom during sexual intercourse. This prevents the bacterium from passing from one person to another.

-Another way of preventing the spread of gonorrhoea is to test people who have had unprotected sexual intercourse (and are therefore possibly infected with gonorrhoea).

-If a person is found to be positive for gonorrhoea, they
should be given antibiotics to kill the bacteria.

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21
Q

Describe how Malaria is Spread

A

-Malaria is a communicable disease caused by a protist.

-However, unlike many other diseases, malaria cannot be passed directly from person to person. Instead, the malaria pathogen is spread via mosquitoes.

-First the mosquito bites an infected person. Blood and the malaria pathogen pass into the mosquito.

-The mosquito then bites an uninfected person, passing on the pathogen.

-Because the mosquito carries the pathogen from one person to another, scientists call it a vector.

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22
Q

Describe how Stooping the Vector from Breeding can Prevent the Spread of Malaria

A

-Mosquitoes prefer to breed in areas of still water.

-To stop this, people can find areas of still water and drain them. This includes ponds and swamps. They can also spray areas of still water with insecticides to kill the mosquitoes.

-However, these methods have not been fully successful in preventing malaria. This is because it is virtually impossible to find every single area of still water.

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23
Q

Describe how Mosquito Nets can be Used to Prevent the Spread of Malaria

A

-One way to reduce the spread of malaria is to prevent mosquitoes from biting people.

-Many people are bitten while they sleep so to prevent that, they should sleep under a mosquito net.

-Because mosquitoes cannot penetrate the net, they cannot bite the person sleeping.

-If we soak the mosquito net in insecticide, then any mosquito which lands on the net will also be killed.

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24
Q

Describe how Tobacco Mosaic Virus Affects Plants

A

-Tobacco mosaic virus is a virus and is not specific to one plant. It is spread through the contact of diseased plant material and insects.

-When plants are infected with TMV, their leaves develop a patchy discolouration. Patches of the leaves have less chlorophyll that normal.

-This means that the rate of photosynthesis is lower than it should be. Because the plant is photosynthesising less than it normally would, the plant grows more slowly.

-It can be prevented by growing resistant strains of plants.

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25
Q

Describe how Rose Black Spot Affects Plants

A

-Rose black spot is a communicable plant disease which is caused by a fungus. This is spread from plant to plant via water or the wind.

-When a plant becomes infected, the leaves develop spots which are black or purple. Eventually, infected leaves turn yellow and fall off the plant.

-This causes the plant to have a reduced rate of photosynthesis. Because of this, plants which are infected with rose black spot will grow more slowly than plants which are not infected.

-We can treat infected plants by removing infected leaves and destroying them so that the fungus can’t spread to other plants. We can also spray the plant with fungicides.

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26
Q

Describe how the Skin Forms a Layer of Protection Against Pathogens

A

-The outer part of the skin consists of a layer of dead skin cells. This is a thick barrier which is difficult for pathogens to
penetrate.

-The skin is also covered in a layer of sebum which can kill bacteria.

-If the skin is damaged then pathogens can enter. To prevent this, the damaged area rapidly forms a scab to block the entry of pathogens.

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27
Q

Describe how the Respiratory System Prevents the Entry of Pathogens

A

-The interior of the nose is covered with hairs and mucus. These trap pathogens that are breathed in, before they can pass down into the lungs.

-The trachea and bronchi are covered in cilia. Cilia are tiny hairs which line the surface of the trachea and bronchi. The cilia are covered with a thin layer of mucus.

-When we inhale pathogens, they stick to the mucus. The cilia then waft the mucus upwards towards the throat, where the mucus is swallowed into the stomach.

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28
Q

Describe how the Digestive System Prevents the Entry of Pathogens

A

-The mouth contains helpful bacteria that get rid of pathogens. Saliva also helps to remove unfriendly bacteria and clean the mouth.

-The stomach contains hydrochloric acid which kills any pathogens that enter on our food.

-The large intestine contains friendly bacteria that stops pathogens from growing.

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29
Q

Describe the Process of Phagocytosis

A

-First, the white blood cell is attracted to chemicals released by the pathogens. This causes the white blood cell to move towards the pathogens.

-The white blood cell attaches itself to the pathogen. The white blood cell now surrounds the pathogen with cytoplasm and ingests the pathogens.

-The white blood cell now uses enzymes to destroy the pathogens.

30
Q

Describe how Antibodies Protect Against Pathogens

A

-Pathogens contain antigens on their surface. When white blood cells detect these antigens, they produce antibodies in response.

-The antibodies lock onto the antigens, rendering them useless whilst other white blood cells now destroy them.

-Antibodies are specific to a particular pathogen and can be produced very quickly should the pathogen re-enter the body.

31
Q

Explain why White Blood Cells that Produce Antibodies Need Lots of Ribosomes

A

-Antibodies are protein molecules.

-Proteins are made on ribosomes so white blood cells that produce antibodies have a large amount of ribosomes.

32
Q

Describe how Antitoxins Protect Against Bacteria

A

-Bacteria can produce toxins that are harmful to the body.

-White blood cells detect the toxins and produce antitoxins.

-The antitoxins neutralise the effects of the toxins.

33
Q

Explain how a Vaccination Makes a Person Immune from Disease

A

-A small amount of a dead or inactive pathogen is introduced to the body.

-This stimulates white blood cells to produce antibodies.

-The white blood cells undergo mitosis to produce lots of identical copies.

-If the pathogen enters the body, antibodies are rapidly produced, preventing infection.

34
Q

Describe how the Number of Antibodies Changes After Vaccination and After the Pathogen Enters the Body

A

-After vaccination, the number of antibodies rises fairly slowly and not to a very high level.

-However, after the live pathogen enters the body, the number of antibodies rises much more rapidly and to a
much higher level.

-This is because after vaccination, the white blood cells undergo mitosis.

-This means that there is a larger number of white blood cells producing antibodies after the live pathogen has entered the body.

35
Q

Describe how Herd Immunity Works

A

-When the entire population is vaccinated, no-one can be infected with the pathogen.

-If a new (unvaccinated) person enters the population, they will not be infected even though they have not been
vaccinated.

-That is because there is no-one in the population who can infect them.

36
Q

Describe the Development in the Use of Antibiotics

A

-In the 1940s, antibiotics were discovered. The first antibiotic that was commonly used was penicillin.

-Antibiotics kill bacteria inside the human body but they do not harm body cells.

-Over time, certain antibiotics were overused and stopped working. The bacteria had evolved and become resistant to
the antibiotic.

-To avoid antibiotic resistance, doctors now use specific antibiotics for specific bacterial pathogens. Doctors are also careful to never prescribe antibiotics for viral infections.

37
Q

Explain why Doctors Cannot Prescribe Just Painkillers to Treat Painful Bacterial Diseases

A

-Painkillers stop pain.

-However, they only relieve the symptoms of a disease but do not stop the actual disease itself.

-Painkillers have no effect on pathogens.

38
Q

Explain why it is Difficult to Develop Drugs to Treat Diseases Caused by Viruses

A

-Viruses can only reproduce when they are inside a host cell.

-This means that it is very difficult to develop drugs to treat diseases caused by viruses without also damaging the host
cell.

39
Q

Describe the Function and Origin of Different Drugs

A

-Penicillin is an antibiotic used to treat bacterial infections. It was extracted from penicillium mould.

-Aspirin is used as a painkiller and to lower fever. It was developed from a chemical in willow trees.

-Digitalis is used to treat heart conditions. It was developed from a chemical found in foxgloves.

40
Q

Explain what is Being Investigated when Testing for: Toxicity, Effectiveness and Dosage

A

Toxicity- This investigates whether the drug is toxic to humans or whether it is safe to use.

Effectiveness- This investigates whether the drug is an effective treatment for the disease which the drug is designed to target.

Dosage- This investigates the best dose to treat the disease with the minimum amount of unwanted side effects.

41
Q

Describe what Happens During Preclinical Trials

A

-During preclinical testing, medicines are not tested on humans. They are tested in cells, tissues (for example samples of muscle tissue) or on live animals.

-During the preclinical stage, we do not know how toxic the drug is to humans. This means that at this stage, the drug is not given to any humans.

The preclinical trials test efficacy (whether the drug works) and toxicity.

42
Q

Describe what Happens During the First Stage of Clinical Testing

A

-In the first stage, a very low dose of the drug is given to healthy volunteers.

-This is to make sure that it doesn’t have any harmful side effects when the body is working normally.

-At the start of the trial, a very low dose of the drug is given and this is gradually increased.

43
Q

Describe what Happens During the Second Stage of Clinical Testing

A

-During the second stage, the drugs can be tested on those who are suffering from the illness.

-The optimum dose is found- this is the dose that is most effective and has few side effects.

44
Q

Explain the Purpose of Placebo

A

-Placebo is used to test how well the drug works. People are randomly placed into two groups. One is given the new drug and the other is given a placebo.

-The placebo looks like the treatment but contains no drug. This is so the doctor can see the actual difference that the drug makes.

-This allows for the placebo effect- when a patient expects the treatment to work and so feels better, even though the treatment isn’t doing anything.

45
Q

Describe what Happens During a Double Blind Trial

A

-In a double-blind trial, one group receives the active drug and another group receives the placebo.

-The placebo looks exactly like the treatment but does not contain any active drug.

-Neither the patient nor the doctors know who is receiving the drug or the placebo.

-This is to prevent bias for example if the doctors gave more attention to patients that they knew were receiving the active drug

46
Q

What Happens to the Drug After it is Tested but Before it is Released

A

-The results of drug testing and drug trials aren’t published until they’ve been through peer review.

-This helps to prevent false claims.

47
Q

Describe how Monoclonal Antibodies are Produced

A

-First a mouse is injected with the antigen that we are making monoclonal antibodies against.

-All of the lymphocytes are then extracted from the mouse.

-Lymphocytes cannot undergo cell division by mitosis. So in this stage, we fuse (join) our lymphocyte with a tumour cell. Tumour cells can undergo mitosis indefinitely.

-We have now produced a cell called a hybridoma. These produce antibodies and can undergo mitosis indefinitely. We select the hybridoma producing the antibody that we want.

-We now allow our hybridoma to undergo mitosis. This produces a clone of identical hybridoma cells, all producing only the antibody that we want.

-We now collect and purify a large amount of our monoclonal antibody.

48
Q

Explain how the pregnancy test strip works to show a positive result.

A

-As urine passes through the reaction zone, HCG hormone binds to the mobile HCG antibody in the reaction zone. There are lots of antibodies so some remain free.

-As the urine passes up the stick, HCG hormone binds to the immobilised HCG antibodies in the results zone.

-The other antibodies which do not attach to HCG
bind to antibodies in the control zone.

-The enzymes on the antibodies react with a blue dye in both the control and result zones. This forms a blue line, showing a positive result.

49
Q

Describe what a Woman will See on a Pregnancy Test if She is Not Pregnant

A

-If a woman is not pregnant then she will see a blue line only in the second panel.

-The woman’s urine will not contain any HCG. This means that when she urinates on the test strip, all the antibodies will remain free.

-These antibodies now make their way down the test strip.
The antibodies pass straight through the first panel without stopping (as they are not attached to HCG). This means that no line forms in the first panel.

-The antibodies continue to the second panel where they attach to the immobilised antibodies and form a blue line.

50
Q

Explain the Purpose of the Second Panel in a Pregnancy Test

A

-The second panel is a control. This shows that the test works.

-If the test were defective and a pregnant woman urinated on this test, then no lines would appear in either panel.

-This would show her that the test is defective.

51
Q

State Three Advantages of Pregnancy Tests based on Monoclonal Antibodies

A

Pregnancy test kits based on monoclonal antibodies are cheap, easy to use and accurate.

52
Q

Describe how Monoclonal Antibodies can be Used to Diagnose Disease

A

-Monoclonal antibodies are used to diagnose disease. For example, they can be used to detect pathogens in the blood.

-They can also detect whether the levels of hormones are too high or low.

-By attaching the antibody to a fluorescent dye, we can find the location of specific molecules in tissues or cells.

53
Q

Describe how Monoclonal Antibodies can be Used to Treat Cancer

A

-Cancer cells produce specific antigens on their surface.

-We can make monoclonal antibodies which attach to these cancer specific antigens. We can make the monoclonal antibodies so that they are attached to a toxin or to a
radioactive element.

-When we inject the antibodies into the patient’s body, the antibodies will attach to the cancer cells. The toxin or the radioactive element will now kill the cancer cells.

54
Q

Describe the Advantage of Using Monoclonal Antibodies to Treat Cancer

A

-In chemotherapy, a cancer patient is given a toxic drug which is designed to kill cancer cells. However, chemotherapy drugs are also toxic to normal body cells. This means it can be very damaging to the patient’s body.

-However, if we attach the toxic drug to a monoclonal antibody, then this drug is delivered exactly to the cells that we are trying to kill. This makes it much less toxic to the rest of the body.

-In radiotherapy, the patient is given a powerful dose of radiation. This will kill cancer cells but also damage normal body cells, producing unwanted side effects.

-However, with monoclonal antibodies, the radiation is delivered specifically to the cancer cells. Again, this reduces the damaging effects on the rest of the body.

55
Q

Explain why There are Only a Few Drugs Based on Monoclonal Antibodies

A

Clinical trials using monoclonal antibodies have produced some very severe side-effects such as changes in blood pressure, breathlessness and headaches.

-It is very expensive for the NHS to produce safe and consistent monoclonal antibodies.

-It is also difficult to get the right antibodies and attach a compound to it.

56
Q

Describe how Aphids can Act as Vectors for Infectious Diseases

A

-Aphids insert their sharp mouthpieces into the phloem of plants and extract nutrients including sugars (and also amino acids).

-When a plant is covered with hundreds of aphids,
then a very large amount of the sugars produced by photosynthesis are taken by the aphids.

-This means that the ability of the plant to release energy by respiration is reduced so the growth of the plant slows.

-Aphids can be destroyed with chemical pesticides or biological pest control e.g. ladybirds to control the aphid population.

57
Q

Describe the Threat Nematode Worms and Insect Larvae Pose to Plants

A

-Nematode worms and insect larvae live in the ground and feed on or in plant roots.

-This prevents the roots from absorbing water and mineral ions effectively.

-Therefore, the plant fails to grow.

-To prevent this, a farmer can rotate crops or use pesticides.

58
Q

State the Symptoms Plants can Show When They Have a Disease

A

-Discolouration (e.g. the leaves changing colour)
-Spots on leaves
-Stunted growth
-Decay / rot.
-Growths.
-Malformed stems or leaves

59
Q

Describe how Gardeners can Use Symptoms to Diagnose which Disease a Plant Has

A

-Once a gardener has identified symptoms of a disease in a plant, they can identify the disease by using a garden manual or a website.

-If they think that the disease is caused by a pathogen then they can send a sample of the plant to a laboratory to identify it.

-The laboratory could use a testing kit based on monoclonal antibodies to identify the pathogen.

60
Q

Describe the Effects on a Plant of a Nitrate Deficiency

A

-Plants need a good supply of nitrate ions to convert sugars made in photosynthesis to proteins for growth.

-If there is a nitrate deficiency, amino acid synthesis is reduced so protein synthesis will be limited. Hence, growth will be slowed.

-To prevent this, fertilise plants (e.g. NPK or compost).

61
Q

Describe the Effects on a Plant of a Magnesium Deficiency

A

-Plants need magnesium ions to make chlorophyll for photosynthesis.

-If there is a magnesium deficiency, chlorophyll synthesis reduced so the leaves lose their green colour and turn yellow/white. This colour change is called chlorosis.

-This means less light is absorbed so the plant cannot photosynthesise. This means less glucose is produced so growth is slowed.

-Prevention includes using fertiliser (but not NPK as it does not include magnesium).

62
Q

Describe how the Stem of a Plant Acts as a Physical Barrier Against Disease

A

-The stems of trees are covered in bark. Bark consists of a very thick layer of dead cells.

-If a pathogen manages to infect bark, it is unlikely to be able to penetrate through to the living tissue further in the stem.

-Over time, the outer layer of bark falls off so any pathogens on the bark will also come off the plant.

63
Q

Describe how the Leaves of a Plant Acts as a Physical Barrier Against Disease

A

-The top surface of a leaf is covered by the waxy cuticle. This protects the leaf from entry by pathogens.

-Many plant pathogens can be transmitted in water droplets falling onto leaves. However, even if this happens, many pathogens would struggle to pass through the waxy cuticle.

64
Q

Describe how the Individual Cells of a Plant Acts as a Physical Barrier Against Disease

A

-All plant cells are protected by a cellulose cell wall.

-This makes it very difficult for pathogens such as viruses to penetrate and enter a plant cell.

65
Q

Describe Chemical Defences that can be Used on or by Plants

A

Insecticides- these are chemicals naturally produced by plants to kill insects as well as insect eggs and larvae e.g. nicotine.

Insect Repellents- repel the insect vectors that carry disease e.g. citronella oils and extracts from lemon grass that repel mosquitos.

Cyanide- in built defence system that when activated releases hydrogen cyanide to ward off insects and fungi.

66
Q

How does the Geranium Plant Defend Itself

A

-Many plants contain poisons which deter animals which might eat the plant (for example bitter chemicals which make the plant very difficult for a herbivore to eat).

-Certain species of geranium contain a chemical which temporarily poisons beetles which have eaten the plant.

-While the beetles are paralysed, they are more likely to be eaten themselves by predators. This protects the plants from the beetles.

67
Q

Describe how Mechanical Defences such as Thorns and hairs protect Plants

A

-Thorns are physically painful for many herbivores to eat.

-Hairs can irritate the mouth of a herbivore causing it to cough or spit out the plant.

-Over time, herbivores learn not to eat these plants.

68
Q

Suggest how Drooping Leaves Defend a Plant

A

-When mimosa leaves are touched, they rapidly droop.

-It is thought that this might deter herbivores by shocking them as herbivores are not used to plants which move suddenly.

-It is possible that by drooping, the leaves cause any beetles to fall off the plant, preventing an attack.

69
Q

Describe how White Dead Nettles Deter Herbivores

A

-White dead nettles have evolved to look very much like stinging nettles.

-It is thought that herbivores might not eat white dead nettles as they would mistake them for stinging nettles and want to avoid being stung in the mouth.

70
Q

How is the Nettle Adapted for Defence

A

-Nettles have stinging hairs.

-These harm herbivores and stops them from eating the nettle.

-In turn, less of the plant is damaged.