Infection And Laboratory Biosafety

Question 1

What is the fundamental objective of Biosafety?

Answer 1

For the containment of potentially hazardous biological agents and toxins.

Question 2

Who developed the logo of Biosafety?

Answer 2

Charles L. Baldwin and Robert S. Runkle in 1966

Question 3

The logo of Biosafety may be _____, ___________ _______, or ______-___

Answer 3

It may be black, fluorescent orange, or orange-red.

Question 4

For the background, it is _______, ________ _______ needed.

Answer 4

It is optional, sufficient contrast is needed.

Question 5

Device (Primary containment) that encloses a working area to protect workers from aerosols exposure and/or infections disease agents.

Answer 5

The Biosafety Cabinet

Question 6

Standard device in the academic and clinical laboratory to contain hazardous biochemical agents and products.

Answer 6

Biosafety Cabinet

Question 7

How is biosafety cabinet air being sterilized?

Answer 7

• Heat
• UV light
• High-Efficiency Particulate Air

Question 8

HEPA filter remove particles larger than ___ mm.

Answer 8

3 mm

Question 9

• Open-fronted, exhaust fan to allow movement of air going through the open front.

• Allows unsterilized air into the cabinet.

• Sample is exposed to contamination.

• Using HEPA filters.

• Laboratory worker environment only is protected.

Answer 9

Class I BSC

Question 10

Mostly used for BSL 1 agents, can be used for BSL 2 agents.

Answer 10

Class 1 BSC

Question 11

• Protection for Laboratory Worker, Environment, and Sample.

• Mostly BSL 1 & 2 agents, provisions available for BSL 3 organisms.

Answer 11

Class 2 BSC

Question 12

• This is the most commonly used BSC.

• For biological and clinically infectious sample.

• For specimens treated with minimal concentration of volatile chemicals.

Answer 12

Class 2, Type A2

Question 13

What is the airflow recirculated and air exhausted in Class 2, Type A2?

Answer 13

70% recirculated and 30% exhausted

Question 14

What is the difference between Class 2A and 2B?

Answer 14

Class 2A air is exhausted TO the room/thimble connection. On the other hand, Class 2B air is exhausted OUTSIDE the facility through a hard duct.

Question 15

• It is a close-fronted BSC with an airtight system, completely separating worker from the sample.

• Glove-box cabinet.

• Highest level of safety to the laboratory.

• Preferred for BSL 4 agents.

Answer 15

Class 3 BSC

Question 16

The interior of the Class 3 BSC is under a ________ pressure.

Answer 16

negative