industrial uses of enzymes Flashcards
what are immobilised enzymes
fixed on inert matrix
example of immobilised enzymes
alginate beads
5 advantages of immobilised enzymes
doesnโt contaminate
small quanity needed
enzymes can be added and removed
greater control over process
greater stability - denature at higher temps
how does glass column work
substrate added at top diffuses into alginate matrix
forms enzyme sub comp
why would you want to decrese flow rate
allow more contact time between enzyme and substrate - more complexes form
why do alginate beads work past normal optimum temp
alginate gel fills and supports enzymes active site maintaining shape of the active site
what do biosnesors do
able to select one type of mol from mixture even in very low concentrations