Induced Pluripotent SC

Question 1

via?

Answer 1

Somatic Cell Nuclear Transfer

Question 2

Describe the process

Answer 2

1. remove the nucleus that contains genetic materials from a somatic cell
2. transfer by injection into unfertilised egg from nucleus been removed
3. newly formed egg starts dividing
   -> create many ESCs
   -> lines are genetically identical
   => prevent immune reaction

Question 3

How it overcomes ethical concerns?

Answer 3

@nuclear reprogramming of somatic cell

• injection of specific T.F, returning to pluripotent state
• transgenes code for STF