Indicators Flashcards
- used in the GasPak
- white when reduced, blue when oxidized
methylene blue
- used in the oxidase test
- dark blue/maroon, oxidized
- no color change, reduced
- positive
- negative
p-amino-dimethylalinine monohydrochloride
- used in carbohydrate broth, KIA agar
- yellow when acidic
- red/pink/orange when basic
phenol red
- used to determine if nitrite is in a solution
- turns red if present, colorless if not
Nitrate A and Nitrate B reagents
- red/pink in acidic environments
- yellow in basic environment
neutral red
bile salts and crystal violet; they are nutritionally fastidious
what are selective components of macconkey agar and why do they inhibit G+ growth
blue green pigment that is water soluble and tested for by Pseudosel agar
Pyocyanin
A quaternary ammonium compound that inhibits growth of other pseudomonas species
Cetrimide
- lime-green pigment produced by pseudomonas species
- best observed under UV light
- tested for by Pseudomonas Agar F
Fluorescein
- forms red ring around top of broth when indole is present
- used in the indole test
Kovac’s Reagent
- red/dark orange when acidic
- yellowish orange when neutral
Methyl Red
- two reagents used in VP test
- form red layer in upper portion of the broth when reacting with diacetyl
Barritt’s A and Barritt’s B reagents