independent study 2

Question 1

Four forms of post-translational modifications

Answer 1

phosphorylation
ubiquitination
acetylation
sumoylation

Question 2

factors that impede phosphoproteomic studies

Answer 2

isolation
large vacuole

Question 3

Four challenges in phosphoproteomics

Answer 3

• identification of protein substrates for diff kinases
• determine whether kinases in the same phosphoproteome
• confirm specificity between kinase and substrate
• prove substrate and kinase in same cell

Question 4

Define a defense-related protein and say how the post-translational modification is detected

Answer 4

R-Protein induced by Pattern Triggered Immunity after detecting residues like cutin from foreign invaders, DAMPS pathway triggered