increasing mitochondrial membrane phospholipid content lowers enzymatic activity of ETC Flashcards
fusion of mitochondria from healthy cardiac muscle with cardiolipin or dioleoylphophatidylcholine results in a 2-6 fold increase/decrease in the activity of ETC complexes 1, 2, and 4
2-6 fold decrease in activity
lipid soluble electron carrier of the mitochondrial respiratory chain
ubiquinone
water soluble electron carrier of the mitochondrial respiratory chain
cytochrome c
Percent of PC, PE, and CL in mitochondrial membrane respectively
44% PC, 34% PE, 14% CL
delipidation of isolated prokaryotic complex 3 reversibly activated/inactivated the enzyme
inactivated the enzyme
the structural integrity of complex 1 is dependent on ___ occupying two high affinity phospholipid binding sites
cardiolipin
cardiolipin effect on complex 2
promotes dimerization and activity
broader effect of CL on ETC
facilitates organization of individual components into higher order assemblies
cardiovascular disease and type 2 diabetes have been associated with increases/decreases in mitochondrial CL
decreases in mitochondrial CL
effect of fusion of PC/CL vesicles to CL depleted mitochondria had this effect?
restored activity
effect of fusion of PC/CL vesicles to healthy mitochondria had this effect?
no effect
increased/decreased lipidation of isolated mitochondria selectively reduces the enzymatic activity of complex 1,2, and 4
increased lipidation
increased/decreased lipidation of isolated mitochondria has a detrimental impact on the rate of metabolite transfer (ubiquinone) and electron flux through complex 1+3 and 2+3
increased lipidation