increasing mitochondrial membrane phospholipid content lowers enzymatic activity of ETC Flashcards

1
Q

fusion of mitochondria from healthy cardiac muscle with cardiolipin or dioleoylphophatidylcholine results in a 2-6 fold increase/decrease in the activity of ETC complexes 1, 2, and 4

A

2-6 fold decrease in activity

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2
Q

lipid soluble electron carrier of the mitochondrial respiratory chain

A

ubiquinone

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3
Q

water soluble electron carrier of the mitochondrial respiratory chain

A

cytochrome c

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4
Q

Percent of PC, PE, and CL in mitochondrial membrane respectively

A

44% PC, 34% PE, 14% CL

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5
Q

delipidation of isolated prokaryotic complex 3 reversibly activated/inactivated the enzyme

A

inactivated the enzyme

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6
Q

the structural integrity of complex 1 is dependent on ___ occupying two high affinity phospholipid binding sites

A

cardiolipin

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7
Q

cardiolipin effect on complex 2

A

promotes dimerization and activity

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8
Q

broader effect of CL on ETC

A

facilitates organization of individual components into higher order assemblies

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9
Q

cardiovascular disease and type 2 diabetes have been associated with increases/decreases in mitochondrial CL

A

decreases in mitochondrial CL

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10
Q

effect of fusion of PC/CL vesicles to CL depleted mitochondria had this effect?

A

restored activity

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11
Q

effect of fusion of PC/CL vesicles to healthy mitochondria had this effect?

A

no effect

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12
Q

increased/decreased lipidation of isolated mitochondria selectively reduces the enzymatic activity of complex 1,2, and 4

A

increased lipidation

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13
Q

increased/decreased lipidation of isolated mitochondria has a detrimental impact on the rate of metabolite transfer (ubiquinone) and electron flux through complex 1+3 and 2+3

A

increased lipidation

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