Impregnation and Embedding

Question 1

Process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill all the tissue cavities, thereby giving a firm consistency to the specimen.

Answer 1

Tissue impregnation

Question 2

Routinely used tissue impregnation

Answer 2

Paraffin wax impregnation

Question 3

Simplest, most common, best embedding medium (24hrs)

Disadvantage:
Specimen brittles when overheated; tissue shrinks and hardens when prolonged

Answer 3

Paraffin wax impregnation

Question 4

Temperature of paraffin oven/ incubator

Answer 4

55 to 60 degrees Celsius

Question 5

Melting point of common waxes

Answer 5

45, 52, 56, and 58 degree Celsius

Question 6

Variations in Performance of Paraffin Wax Impregnation:
Utilizes an “autotechnicon”
For rapid diagnosis and less technicalities
2-3 changes of wax (fix pure paraffin wax) is required

Answer 6

Automatic processing

Question 7

Variations in Performance of Paraffin Wax Impregnation:
Utilizes a negative atmospheric pressure inside an embedding oven
Provides the fastest results (25 to 75%)

Answer 7

Vacuum embedding

Question 8

Alternative for Paraffin Wax:
Pure paraffin and synthetic plastic polymers
MP: 56 to 57 degree Celsius
Variations:
*Embeddol
*Bioloid
*Tissue mat

Answer 8

Paraplast

Question 9

Alternatives for Paraffin Wax
Harder than paraffin
MP: 46 to 48 degree Celsius

Answer 9

Ester Wax