Impregnation and Embedding

Question 1

Process whereby the clearing agent is completely removed from the tissue and is replaced by a medium that will completely fill all the tissue cavities

Answer 1

IMPREGNATION or INFILTRATION

Question 2

• Process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is ten allowed to solidify

Answer 2

EMBEDDING

Question 3

Embedding is also known as

Answer 3

CASTING or BLOCKING

Question 4

Medium used in impregnation will be the same for embedding

Answer 4

TRUE

Question 5

• Simplest, most common and best embedding medium used for routine tissue processing

Answer 5

PARAFFIN WAX

Question 6

• Defined as obtaining a continuous ribbon of sections from a paraffin block and placing all the sections on multiple slides

Answer 6

Serial Sectioning

Question 7

How long may Tissue blocks and unstained mounted tissue sections may be stored in paraffin after impregnation without tissue distortion

Answer 7

INDEFINITE PERIOD

Question 8

• Prolonged impregnation will cause

Answer 8

excessive tissue shrinkage and hardening

Question 9

• Inadequate impregnation will cause

Answer 9

retention of clearing agent
becomes soft and shrunken
blocks crumble or break up when floated in a water bath

Question 10

• Paraffin processing is recommended for fatty tissues

Answer 10

FALSE

Question 11

medium should be in liquid state

Answer 11

Infiltration

Question 12

medium should solidify

Answer 12

Embedding

Question 13

microtome used

Answer 13

Use sledge or sliding microtome

Question 14

melting point of commonly used paraffin

Answer 14

56 degree Celsius

Question 15

recommended temperature for manual infiltration

Answer 15

2-5 degree Celsius above the melting point of the wax

Question 16

temperature where tissues start to become brittle and shrink

Answer 16

temp above 60

Question 17

Manual infiltration has how many changes

Answer 17

2-4 changes, at least 4

Question 18

incubator or paraffin oven is regulated in what temperature

Answer 18

55-60 degree Celsius

Question 19

• In a room temperature (20-24 degree Celsius) what melting point of wax is recommended

Answer 19

54-58 degree Celsius

Question 20

• If the laboratory temperature is between 15-18 degree Celsius what melting point of wax is recommended

Answer 20

50-54 degree Celsius

Question 21

Manual processing: how many minutes of interval

Answer 21

15 mins

Question 22

Manual processing: how long is the tissue immersed in melted paraffin (last)

Answer 22

3 hours

Question 23

• Makes use of an automatic tissue processing machine which fixes, dehydrates, clears, and infiltrates tissues

Answer 23

Automatic processing

Question 24

The tissue basket oscillates up and down in each station at three-second intervals

Answer 24

Oscillating tissue basket

Question 25

automatic processing: beakers for fixation

Answer 25

Beaker I, II, III

Question 26

Automatic processing: beakers for dehydration

Answer 26

Beaker IV, V, VI, VII, VIII

Question 27

Automatic processing: beakers for clearing

Answer 27

Beaker IX, X

Question 28

Automatic processing: how many thermostatically controlled beakers and its regulated temperature

Answer 28

2

regulated at 45 degree Celsius

Question 29

Automatic processing: how many was changes

Answer 29

2-3 changes

Question 30

accelerates and improves tissue penetration giving rise to more consistent results

Answer 30

constant tissue agitation