Immunophenotype Markers Flashcards
1
Q
HLA-DR
A
- Marker of immaturity that is very common in leukemias, except:
- Almost absent in APML FAB M3 (10%)
- Less common in AMKL FAB M7 (30%)
- Not common in T-ALL
2
Q
TDT
A
- Marker of lymphoid immaturity
(B- and T-ALL)
3
Q
CD10
A
- B-ALL marker, aka. common ALL antigen (CALLA)
- Not expressed in KMT2A-rearranged B-ALL
- Also positive in 1/3 of T-ALL
4
Q
CD15
A
- Classical Hodgkin lymphoma
- Typically, CD15 and CD30 positive, CD45 negative, down-regulated B cell markers
5
Q
CD19
A
- Pan B cell marker
- Often aberrantly expressed in AML with a t(8;21) translocation
6
Q
CD20
A
B cell marker
7
Q
CD22
A
- B-ALL marker
- Typically, only found in mature B-cells in non-ALL settings
8
Q
CD30
A
- Classical Hodgkin lymphoma
- Typically, CD15 and CD30 positive, CD45 negative, down-regulated B cell markers
- PMBL
- ALCL
9
Q
CD45
A
- NLPHL (CD45 positive, CD15 negative, CD30 negative)
- Retains some other B cell markers
10
Q
CD79
A
Pan B cell marker
11
Q
CD3
A
T cell marker
12
Q
CD4
A
- T helper cell marker
- Binds to MHC class II expressed on antigen-presenting cells
13
Q
CD43
A
Deficient or abnormal glycosylated in T cells in WAS
14
Q
CD5
A
- T cell marker
- Expressed on a small subset of B cells (B1a cells)
15
Q
CD7
A
T cell marker
16
Q
CD8
A
- Cytotoxic T cell marker
- Binds to MHC class I expressed on all healthy cells as part of self-surveillance
17
Q
CD13
A
- Pan-myeloid marker, positive in AML
- Aberrantly expressed in ETV6-RUNX1 and BCR-ABL1 ALL
18
Q
CD14
A
- Monocytic marker
19
Q
CD163
A
- Rosai-Dorfman disease and Juvenille xanthogranuloma
- Monocyte and macrophage marker
- Identifies emperipolesis
20
Q
CD1a
A
LCH Marker
21
Q
CD207
A
LCH Marker
22
Q
CD33
A
- Pan-myeloid marker, positive in AML
- Aberrantly expressed in ETV6-RUNX1 and BCR-ABL1 ALL
23
Q
MPO
A
- Pan-myeloid marker, positive in AML
- Not expressed in AMKL FAB M7
24
Q
CD11b
A
- Loss of CD11a/b in LAD type 1
- Forms the macrophage-1 antigen receptor, Mac-1, when bound to CD18
25
Q
CD18
A
- Loss of CD18 in LAD type 1 due to mutations in ITGB2 (ß2-integrins)
- ß2-integrins are an essential component of CD18
- Forms the macrophage-1 antigen receptor, Mac-1, when bound to CD11b
26
Q
CD235A
A
- Erythroid marker (aka. glycophorin A), positive in AML FAB M6
27
Q
CD55
A
- Absent in PNH, mutations in PIGA lead to a deficiency of CD55/59
- Allows complement (C3) to activate the alternative pathway and mediate hemolysis
- Patients also at risk for thrombosis
28
Q
CD59
A
- Absent in PNH, mutations in PIGA lead to a deficiency of CD55/59
- Allows complement (C3) to activate the alternative pathway and mediate hemolysis
- Patients also at risk for thrombosis
29
Q
CD41a
A
- Platelet marker, positive in AMKL FAB M7
30
Q
CD42
A
- Platelet marker, positive in AMKL FAB M7
31
Q
CD61
A
- Platelet marker, positive in AMKL FAB M7
32
Q
GP1B
A
- Absent in Bernard-Soulier syndrome
- Binds von Willebrand factor
- Pathologically higher affinity of GP1b in von Willebrands Disease type 2B (platelet type)
33
Q
GP2b/3a
A
- Deficient in **Glanzman’s **thrombasthenia
- Platelet marker that binds to fibri
34
Q
CD107a
A
- Degranulation marker to measure natural killer cell function
- Useful in the diagnosis of HLH
35
Q
CD56
A
- Neuroblastoma and natural killer cell marker
- Neural cell adhesion molecule (NCAM)
36
Q
CD99
A
- Ewing sarcoma and PNET
37
Q
Cytokeratin
A
- Synovial carcinoma (as well as some other sarcomas)
38
Q
Desmin
A
Rhabdomyosarcoma
39
Q
Muscle Specific Actin
A
Rhabdomyosarcoma
40
Q
MyoD
A
Rhabdomyosarcoma
41
Q
Myogenin
A
- RMS, diffusely positive in ARMS, patchy positive in ERMS
42
Q
NSE
A
- Neuroblastoma and sometimes Ewing Sarcoma
43
Q
Synaptophysin
A
- Neuroblastoma and PNET
44
Q
Vimentin
A
- Ewing sarcoma, PNET, and synovial sarcoma marker
