Immunomodulators

Question 1

Define monoclonal antibodies, and describe in principle how they are made.

Answer 1

Made from multiple myeloma cells,

Question 2

Discuss the use of monoclonal antibodies as anti-inflammatory agents.

Answer 2

You can target TNF for example

Question 3

Chimeric Monoclonal antibodies

Answer 3

Fusion of mice and human antibodies

VL and VH domains, but human C domains;

Question 4

Human Monoclonal Antibodies

Answer 4

Completely human antibodies

Question 5

Humanized Monoclonal Antibodies

Answer 5

only the CDR’s of the V domains are from the mouse

Question 6

Define NK cells and ADCC. Discuss the effect of Class I MHC expression levels on susceptibility of target cells to CTL and NK cells, respectively. Describe the mechanism for ADCC.

Answer 6

NK cells are lymphocytes that are larger than others, (arge granular lymphocyte)

No VDJ rearrangement, so not adaptive
Recognizes damaged or stressed cells
ALso as FC receptors for IgG bound to our cells

Question 7

Describe how a monoclonal antibody against a T cell surface molecule could enhance the activity of a CTL.

Answer 7

x

Question 8

Discuss the use of modified (drugs, isotopes) monoclonal antibodies in tumor diagnosis or therapy.

Answer 8

x