Immunochemical Techniques

Question 1

What are immunochemical techniques

Answer 1

Based on the reaction of an antigen with an antibody reaction of an antigenic determinant with the binding site of the antibody immunochemical methods are based on the selective, reversible and non-covalent binding of antigens or antibodies and others employed to detect or quantify either antigens or antibodies

Question 2

Factors controlling immunochemical techniques

Answer 2

Experimental conditions the nature, place of work, type of sample collected
Nature of reagents - the quality is studied standardized and analysed
Sensitivity and selectivity of technique to the particular sample

Question 3

Characteristic and role of immunochemical techniques

Answer 3

Immunochemical methods are processes which utilise the highly specific affinity of an antibody for its antigen they can detect the distribution of a given protein or antigen and tissues or self the methods used for immunochemical analysis are called immunochemical techniques they are highly important in a diagnostic and clinical context as even normal cells with many proteins are altered in a disease state

Question 4

Characteristics and role of immunochemical techniques

Answer 4

Immunochemical methods are processes which utilise the highly specific affinity of an antibody for its antigen they can detect the distribution of a given protein or antigen and tissues or cells the methods used for immunochemical analysis are called immunochemical techniques they are highly important in a diagnostic and clinical context as even normal cells with many proteins are altered in a disease state

Question 5

Characteristics of immunochemical techniques

Answer 5

Simple rapid and robust
Highly sensitive easily automated applicable to regular clinical diagnostic labs
Do not require extensive and easily destructible sample preparation
Do not require expensive instrumentation
Mostly based on simple photo fluoroand luminometric detection
Measurements may be the qualitative or quantitative

Question 6

Roles of immunochemical techniques

Answer 6

Helpful for
Determining the function of newly identified a novel proteins
Analysing the importance of uncharacterized proteins in their natural environment
Determining species or tissues in which the proteins or residues are expressed
Identifying the cell type of substance compartment in which the protein can be found Detecting whether there is any variation in protein expression during the development of the organism
Giving information that may be helpful for diagnosis and treatment