Immunoassays

Question 1

What is an immunoassay?

Answer 1

a test to detect and quantify an antigen in your sample

Question 2

What do antibodies recognize?

Answer 2

Antibodies recognize antigens through specific epitopes

Question 3

Monoclonal

Answer 3

One type of Ab
Recognizes one single epitope

Question 4

Polyclonal

Answer 4

Mixture of Abs bind to the same Ag
Multiple epitopes recongized

Question 5

How can we detect primary Abs?

Answer 5

-Option A: directly bound to fluorescent dyes, compounds, enzymes for detection
-Option B: Primary antibody + secondary antibody that is conjugated with a detection tag

Question 6

Direct methos can avoid ____ and cau be used with

Answer 6

avoid cross-reactivity between 2o, can we use multiple abs from same species

Question 7

Types of detection tags:

Answer 7

Fluorescent tags
Enzyme-linked Ab

Question 8

Sometimes the antigen is in very low quantity in the sample… thus

Answer 8

we need to modify our assay to make it more sensitive signal amplification

Question 9

How can we amplify the signal?

Answer 9

Multiple 2nd Abs can bind to each 1 Ab

Question 10

Immunohistochemistry

Answer 10

Selectively imaging antigens inside cells of tissues

Question 11

Flow cytometry

Answer 11

Detect and measure characteristics of cell pops

Question 12

ELISA

Answer 12

High throughput method of quantitatively measuring protein concentration compared to standard

Question 13

Immunoblotting

Answer 13

Western blot - detecting specific proteins

Question 14

Southern blot

Answer 14

detecting specific DNA seqs

Question 15

Northern blot

Answer 15

Detecting specific RNA seqs

Question 16

Northern blot

Answer 16

Detecting specific RNA seqsImmunprecipitation

Question 17

immunoprecipitation

Answer 17

A technique of precipitating an antigen out of solution using a specific Ab