Immunity Flashcards
3 things that promote phagocytosis
Common bacterial cell wall components (PAMPs)
C3b complement component
Fc region of antibodies
3 types of PAMPs
Common cell wall structures e.g., LPS and peptidoglycans
Bacterial metabolic processes
Heat-shock proteins (released by stressed cells)
Acute phase proteins
Produced early in infections and tissue injury in response to early alarm mediators
Act to enhance host resistance, minimise tissue injury and promote resolution and repair of inflammatory lesions
Effector cells
B cells
CD8 cells
Natural Killer cells
Regulator cells
CD4 cells
Th1, Th2, Treg + T17
Specialised APCs of the: Skin Lungs Blood Liver Gut
Skin – Langerhans cells (transport to regional nodes)
Lungs – Alveolar macrophages (transport to spleen or regional nodes)
Blood – Blood monocytes (transport to spleen)
Liver – Kupffer cells (transport to spleen or regional nodes)
Gut – Epithelial M cells (transport to Peyer’s patches)
Process of antigen presentation by APC e.g., skin wound
Multiplying bacteria uptaken into Langerhans cells of skin
Dendritic cells bind bacteria and transport it through lymphatics
Antigen enters lymph node, followed by small naive lymphocytes from the bloodstream
T cells migrate to paracortical areas of nodes and B cells migrate to follicles
Lymphocytes that recognise the antigen are activated and stop recirculating
Lymphocytes that do not recognise the antigen leave via afferent lymphatic vessels
After several days activated lymphocytes leave the efferent lymphatic vessel as effectors
Meanwhile, antigen is held in lymph node and antigen-specific cells are recruited
Endogenous pathway of antigen processing
Used for peptides derived from cytoplasmic proteins, like in viral infections Cytosolic proteins degraded into peptide fragments by proteasomes Peptides produced are unaccessible to class I MHC molecules, which are bound to the TAP-1 transporter complex on the ER membrane Peptides transported into ER lumen by TAP-1 transporter and inspected by TAP-1-bound class I MHC When a peptide binds to class I MHC, the MHC molecule folds around the peptide and is released from TAP-1 to be transported to the cell membrane
Exogenous pathway of antigen processing
Used for peptides derived from ingested material Antigen is taken up from outside the APC into intracellular vesicles Acidification of vesicles activates proteases to degrade antigen into peptide fragments Vesicles containing peptide fragments fuse with vesicles containing class II MHC and peptides with affinity for antigen-binding groove of class II MHC bind to it Bound peptide transported by class II MHC to cell surface
Where are class I MHC molecules expressed?
Virtually all nucleated cells in the body
Therefore most cells can present peptide fragments derived from metabolic breakdown of intracellular infectious processes
Where are class II MHC molecules expressed?
Only on B cells and professional APCs
Therefore only these cells can present peptide fragments of ingested material
Th1 cells have activity against:
Viruses, bacteria and intracellular agents
Th2 cells have activity against:
Parasites, allergies and multicellular agents
The main role of Treg cells is to:
Downregulate other responses/suppress antigen-specific
The main role of Th17 cells is:
Inflammation and mucosa maintenance
4 types of immune conversations
Adhesion molecules
Co-stimulator molecules
Cytokines
Hormones
3 types of adhesion molecules
Selectins
Integrins
Cadherins
Co-stimulators
Surface molecules induced on antigen-presenting cells and lymphocytes
Involved in modulating lymphocyte activation and function
Example of co-stimulators found on B cells
CD40 (which binds to CD40-L on CD4 T cell)
CD40 then upregulates B7 which binds CD28 on the T cell which then releases cytokines
Type I interferons
IFN-a and IFN-B
Proinflammatory cytokines
IL-1, IL-6 and TNF-a
Adaptive immune response cytokines
Il-1, Il-2, IFN-y and IL-4–6
Chemokines
IL-8, MCP-1 and MIP-1a
Haematopoietic cytokines
IL-3, G-CSF, M-CSF, GM-CSF, IL-5 and IL-7
IL-1
Second messenger to activate T cells following contact with antigen
IL-2
Stimulates clonal proliferation of antigen-specific T cells
IFN-y
Causes activation of macrophages, promotes HLA expression and activates NK cells
IL4–6
Important in stimulating growth and differentiation of B cells
IL-8
Influences neutrophil chemotaxis and activation
CSF cytokines
Stimulate proliferation of granulocytes (G) and monocytes (M)
IL-3
Promotes proliferation of all lineages of haematopoietic cells
IL-5
Stimulates eosinophil growth and activation. Also important in allergic responses
IL-7
Stimulates erythroblast and megakaryocyte growth and involved in B cell development
MCP-1 and MIP-1a chemokines
Important in allergic inflammation by stimulating basophils to release histamine
Adhesion molecules
Link cells to other cells
Important in binding lymphocytes to antigen-presenting cells and also in directing lymphocytes and phagocytic cells to parts of the body where they are needed
Co-stimulator molecules
Pass signals between linked cells through surface–surface interactions to trigger or inhibit antigen-specific effects
Cytokines
Soluble glycoproteins that signal cells expressing specific cytokine receptors e.g., lymphocytes following antigen activation
Hormones
Soluble molecules produced by neuroendocrine hormones that modulate the response of antigen-activated cells
3 types of selectins
L-selectin (lymphocytes)
P-selectin (platelets)
E-selectin (endothelial cells)
Integrins
Important in cell–cell adhesion and cell–ECM adhesion
Hold lymphocytes together for activation and hold lymphocytes on capillary wall
Leukocyte adhesion deficiency
Hereditary deficiency of some integrins
Leukocyte trafficking impaired and patients are susceptible to recurrent pyogenic infections
Anergy
Fail-safe mechanism that prevents T cells from responding to self-antigens
Occurs when an antigen is presented to a T helper cell without the required co-stimulator molecule being presented on the APC causing the T cell to become paralysed
Properties of IgM
Large pentamer, confined to bloodstream, does not cross placenta, 3rd most common
Produced early in primary antibody response, good defence against bacterial spread
Efficient agglutinator and complement activator
Properties of IgG
Small monomer, diffuses easily out of blood, crosses placenta, by far the most common Major class in secondary responses Good complement activator, opsonin and Fc receptor-mediated effector mechanisms
Properties of IgA
Defends exterior surfaces
2nd most common
Properties of IgD
Trace amounts
HIghly sensitive to proteolysis
Receptor on virgin, antigen-sensisitve B lymphocytes
Properties of IgE
Trace amounts unless allergy or parasite infection
High affinity Fc receptor on mast cells and basophils
Antibody structure
Four polypeptide chains held together by disulphide bonds and non-covalent interactions Light chains and either kappa or lambda Heavy chains can be one of 5 types with each giving rise to the class of antibody Variable and constant regions on the top of the Y Also an Fc region on the bottom of the Y containing the bottoms of the heavy chains, which are constant regions, a complement-binding region and a hinge region