Immunity Flashcards
What does pathogenic mean
Disease causing
What is the body’s first line of defence?
To prevent entry of the organism
What are two groups defence mechanisms can be grouped into and give a brief summary of each.
1) Non-specific –> Not specific to individual pathogens e.g. phagocytosis
2) Specific –> Distinguishes between individual pathogens. Takes longer to work but provides long term immunity. e.g. Lymphocytes
What are the 4 natural barriers to pathogen entry and explain each.
1) Skin - Tough physical barrier, only effective when unbroken
2) Lysozyme - In tears and sweat, antibacterial which can hydrolyse bacterial cell walls. Tears wash away debris
3) Epithelial lining covered in mucus - Traps pathogens, cilia sweep pathogens back up trachea
4) HCl in stomach acid - Kills pathogens
What is phagocytosis?
Non specific, rapid immune response.
How does phagocytosis work?
During inflammatory response, capillaries become leaky thus plasma seeps into surrounding area. (raises temperature - denatures enzymes). Polymorphs arrive first, macrophages next (develop from monocytes, larger and longer lived) and engulf pathogenic bacteria.
Describe the process of phagocytosis
1) Phagocyte moves towards the pathogen attracted by the chemicals it produces
2) Phagocyte membrane invaginates
3) Pathogen engulfed, now in a phagosome
4) Hydrolytic enzymes within the lysosomes hydrolyse the pathogen
5) Soluble products absorbed
Define antigen
A substance capable of stimulating the production of specific and complementary antibodies
Define antibody
Globular proteins that are specific and complementary to particular antigens that can react with antigens leading to their destruction.
Why do lymphocytes not attack ‘self’ cells
Lymphocytes that are complementary in shape to foetal (self) cells are switched off
What are the two types of lymphocytes
B - Lymphocytes (B-cells)
T - Lymphocytes (T-cells)
B lymphocytes: Formed, develop, type of immune response, nature of immune response
1) Formed - Bone marrow
2) Develop - Bone marrow
3) antiBody-mediated (humoral) immunity
4) Produce antiBodies which respond to antigens found in body fluid - bacterial or viral
T lymphocytes: Formed, develop, type of immune response, nature of immune response
1) Formed - Bone marrow
2) Develop - Thymus gland
3) Cell-mediated immunity
4) Respond to antigen presenting body cells - viral infection
How are lymphocytes activated? Any differences between B and T?
A lymphocytes comes into contact with its complementary antigen, lymphocytes become sensitised. What is produced is different.
Cell mediated immunity - describe how the immune response comes about and describe the immune response.
T-cells stimulated by the body’s own (antigen presenting cells).
T-cells divide by mitosis to produce 4 different cells:
1) Killer T-cells –> Attach to antigens on the surface of the target, release perforin which produces pores on cell surface membrane - death. Hydrolytic enzymes also released
2) Helper T-cells –> Secrete cytokines e.g. interferon which promotes activation of other cells. e.g. B-cells - plasma cells = more antibodies. Promote phagocytosis. Attach opsonins to mark out the pathogens for phagocytosis.
3) Suppressor T-cells –> Secrete cytokines which deactivate B and T cells, thus preventing autoimmune diseases.
4) Memory T-cells –> circulate in body fluid, respond rapidly to future infection by the same pathogen. Rapidly produce T-cells = IMMUNITY.
Antibody mediated immunity - describe how the immune response comes about and describe the immune response.
Targets microorganisms that are in body fluids. Defends the body through the production of antibodies. Specific antigens sensitise specific B-lymphocytes, which divide by mitosis to produce B-plasma and B-memory cells. Plasma cells are short lived, but produce huge numbers of antibodies - neutralise pathogens via antigen-antibody complexes.
Describe the action of antibodies and B-memory cells
1) The antibodies latch onto complementary antigens clumping the bacteria together, this causes agglutination or clumping as an antigen-antibody complex. It is then engulfed by polymorphs and other phagocytes. They can also act as opsonins by attaching to pathogens and marking them out for phagocytosis.
2) Memory cells remain in body fluids. Once stimulated divide rapidly by mitosis to produce vast numbers of plasma cells - Secondary immune response.
What do T cells also attack
Transplanted tissues as they are non self and caner cells
What is passive immunity?
Individuals receiving antibodies form another source
What is active immunity?
Individuals achieve immunity through the production of their own antibodies.
How can you gain passive immunity?
1) Antibodies passing from mother to baby across the placenta / mothers colostrum - crucial for a baby.
2) Antibodies made in another individual, harvested and injected as serum (from convalescing individual)
3) Monoclonal antibodies from another species.
How do you obtain monoclonal antibodies? What are the advantages.
Removal of sensitised and cloned B-Lymphocytes from a mouse that has been infected by a particular antigen. Then hybridise them with cancer cells. Put in a fermented and they divide rapidly.
Produced in large quantities in a lab.
Can produce a single type of antibody
Advantages and Disadvantages of passive immunity
Advantage - Rapid as antibodies are injected
Disadvantage - Temporary