IMMS Flashcards
Label
Where does krebs cycle occur?
Where does electron transport occur?
Where does ATP synthesis occur?
Matrix
Cristae
Inner membrane
What occurs in matrix?
What occurs in cristae?
What occurs inner membrane?
Matrix- krebs
Electron transport- cristae
ATP synthesis- inner membrane
Function mitochondria?
Site respiration and ATP formation
Function ribosomes?
Site protein synthesis
Function rough ER?
Synthesis and process proteins
Function smooth ER?
Enzymes for lipid synthesis
Function golgi apparatus?
Process and package proteins
Function cytoskeleton?
Structural support
Function nucleus?
Genetic info and make ribosomal subunits
Function lysosomes?
Acid hydrolases for digestion and recycling
Function peroxisomes?
FA oxidation
Contain catalse
Label
What is cell membrane made of?
Phospholipid bilayer
Contain:
Cholesterol
Proteins
Glycolipids and glycoproteins
Function of cell membrane? (4)
1) Partially permeable membrane
2) Site membrane receptors
3) Regulate what enter and exit cells
4) Barrier seperate intracellular content from extracellular contents
What does phospholipid contain?
Hydrophilic polar head- phosphate, glycerol
Hydrophobic non-polar fatty acid tail
What are 2 types membrane proteins?
Integral
Peripheral
Name 3 types integral proteins?
1) a helix- recognition receptor
2) Helical bundle- enzymes, transporter receptor
3) Beta barrel- transporter- channel proteins
Name 2 types peripheral proteins?
1) Enzymes
2) Transporter
Give 3 ways that movement across membranes occurs?
1) Simple diffusion
Passive movement particles from region high to low concentration through partially permeable membrane
Small, non-polar
2) Facilitated diffusion
Transport particles through partially permeable membrane down conc gradient by carrier molecule
Large, polar
3) Active transport
Transport particles against conc gradient- require ATP
Proteins, ions, complex sugars
Define homeostasis?
Maintenance of constant internal environment
Define positive and negative feedback?
Positive- amplification of signal
Negative- bring back equilbrium
List 4 types of signaling?
1) Autocrine
2) Paracrine
3) Endocrine
4) Exocrine
Autocrine- where secreted, where act?
Paracrine- where secreted, where act?
Endocrine- where secreted, where act?
Exocrine- where secreted, where act?
Autocrine- cell secrete chemical/hormone into ECF
- act on self
- progesterone
Paracrine- secrete and travel ECF- adj cell
- local cell comm ACh at NMJ
Endocrine- secrete into blood
- long distance signalling
- insulin
Exocrine- secretion via ducts into organs
- salivary and sweat
Give example negative feedback?
Regulation blood calcium lvl
Give example positive feedback?
Oxytocin- childbirth
Clotting factors- coagulation
What are 2 main types hormones?
Steroid
Peptide
What steroid hormones derived from?
Are steroid hormones stored?
Slow or fast
Example
Steroid hormones derived from cholesterol
Formed when needs- not stored
Slow acting
Cortisol
What peptide hormones derived from?
Are peptide hormones stored?
Slow or fast?
Example
Peptide hormones derived from amino acids 3-20
Stored secretary vesicles
Fast acting
What amine hormones derived from?
Amino acids- 2
Fast
Stored
Adrenaline
State 4 ways water is controlled?
1) ADH
2) Thirst
3) Aldosterone
4) Sympathetic ns
What releases ADH?
Posterior pituitary
Does ADH increase or decrease water reabsorption?
Increase water reabsorption in collecting ducts in nephrons of kidney
State one effect aldosterone?
Allow sodium move into blood- increase blood volume
What does arteriole do in response low water conc?
Neurotransmitters from sympathetic nerves in kidney stimulate smooth muscle in afferent arteriole constrict
Less urine formation
Renin release
If aldosterone present is more or less potassium excreted?
More potassium
What decreases blood calcium?
Calcitonin
Name 2 factors increase blood calcium?
PTH
Calcitriol
Draw water distribution 70kg male?
Define hypotonic?
Overhydration
Define hypertonic?
Deydration
List 3 symptoms overhydration?
Cells swell- cell lysis
Headaches, coma
Death
List 3 effects dehydration?
Seizures
Brain damage
Death
State formation
Maltose
Sucrose
Lactose
Glucose + Glucose= Maltose
Glucose + Fructose= Sucrose
Glucose + Galactose= Lactose
What bond joins glucose and glucose?
Glycosidic
What joins amino acids?
Peptide bonds
What type of reaction joins amino acids?
Condensation reaction
Is carboxyl group positive or negative?
Carboxyl- +ve
Amino- -ve
How many amino acids exist?
20
Define
Primary
Secondary
Tertiary
Quaternary
Proteins?
Primary- linear sequence amino acids
Secondary- local folding alpha helix, beta pleated
Tertiary- 3D folding due chain interactions
Quaternary- protein more one amino acid chain
Where does electron transport chain occur?
Cristae of mitochondria
What is function golgi apparatus?
Process and package proteins
Describe phospholipids property?
Polar, hydrophilic head
Non-polar hydrophobic tail
How large polar molecules transported across membranes
Facilitated diffusion
What kind signaling used long distance signalling?
Endocrine
Name mechanism water homeostasis?
ADH, thirst, aldosterone, sympathetic ns
What effect of aldosterone on sodium and potassium?
Increased reabsorption sodium
Increased excretion potassium
How many amino acids are there?
20
Outline how many calories?
Carbohydrates
Protein
Alcohol
Lipid
Carbohydrate- 4kcal/g
Protein- 4kcal/g
Alcohol- 7kcal/g
Lipid- 9kcal/g
Define BMR?
Amount energy needed keep body alive at rest
1kcal/kg body mass/hr
State 5 things that increase BMR?
1) High BMI
2) Hyperthyroidism
3) Fever/infection
4) Pregnancy
5) Exercise
State 4 things decrease BMR?
1) Ageing
2) Female
3) Starvation
4) Hypothyroidism
What process breaks down glucose to release enrgy?
Glycolysis
Where does glycolysis occur?
Take place cytosol`
State glycolysis equation?
What occurs to pyruvate that is produced from glycolysis?
Aerobic
Anaerobic
Aerobic- pyruvate enter citric acid cycle
- undergo oxidative phosphorylation
Anaerobic- pyruvate convert lactate
How much ATP generated in aerobic glycolysis?
How much ATP generated in anaerobic glycolysis?
Aerobic- 38ATP
Anaerobic- 2ATP
Where does Krebs cycle occur (citric acid cycle)?
Mitochondrial matrix
Draw Krebs cycle?
What is rate limiting step?
Isocitrate converted alpha-ketoglutarate
What is rate limiting step enzyme in krebs?
Isocitrate dehydrogenase
What is responsible for FA oxidation?
Peroxisomes break down FA
What is produced during high rates fatty acid oxidation?
Acetyl coA- exceed krebs
What is pyruvate from glycolysis converted into?
Pyruvate converted into acetyl coA
Define ketogenesis?
Ketone bodies made in mitochondrial matrix from acetyl coA (generated in beta oxidation)
Give examples of ketones?
Acetone
Acetoacetate
Can liver use ketones?
No
What muscles can use ketones?
Skeletal muscles
Structure DNA?
Double helix structure
What are nucleotides composed of?
Nitrogenous base + sugar + phosphate
Give 2 examples of purine? What is it?
Adenine
Guanine
2 carbon-nitrogen
Give 2 examples of pyrimidine? What is it?
Thymine
Cytosine
1 carbon-nitrogen
Two types of sugar in nucleotides?
Ribose (RNA)
Deoxyribose (DNA)
What is coding section of gene?
Exon
What is non-coding section of gene?
Intron
State DNA mutation?
Duplication
Deletion
Substitution
Define
Out of frame mutation
In frame mutation
Out of frame- one base deleted- shift sequence BIG ISSUE
In frame- complete codon deleted- milder
Is out of frame or in frame mutation more dangerous?
Out of frame
Shift reading sequence
State 3 forms DNA damage?
Chemicals
UV
Radiation
Define missense mutation?
Single nucleotide change results in codon coding for diff amino acid
Lead silent mutation
Define nonsense mutation?
Point mutation produces stop codon- incomplete protein
Explain expansion of tri-nucleotide repeat?
Example?
Triple repeat repeated svl times in first part of coding seq
Normal- 15-20
Huntingtons CAG- greater 36
Anticipation- more repeats- earlier onset pass generation
Name 3 types RNA?
Messenger mRNA
Ribosomal rRNA
Transfer tRNA
State DNA and RNA:
Functiono
Structure
Length
Sugar
Bases
Location
Which way is template strand read in DNA transcription?
Which way is strand formed?
3’ to 5’
5’ to 3’