IM

Question 1

Other name of infectious mononucleosis

Answer 1

Torrid/French Kissing Disease or Glandular Fever

Question 2

Most common transmission of IM

Answer 2

Exchange of saliva through kissing

Question 3

What kind of virus is IM?

Answer 3

DNA Virus

Question 4

The infection that is most common during the adolescent stage and early adulthood in women

Answer 4

Epstein Barr Virus

Question 5

EBV infects what type of lymphocyte?

Answer 5

B-lymphocyte

Question 6

EBC inhabits at?

Answer 6

nasopharyngeal tissues

Question 7

EBV can be asymptomatic?
True or False

Answer 7

True

Question 8

Other name of Downey cells

Answer 8

Atypical or reactive lymphocyte

Question 9

Enlarged lymphocytes affected by EBV with characteristics atypical nuclei

Answer 9

Downey cells

Question 10

Symptoms of IM are similar with?

Answer 10

Symptoms of early stage of AIDS
- fever
-fatigue
-sore throat
-swollen lymph glands

Question 11

Cell associated with IM that is oversized, has nuclei and cytoplasm surrounded by RBCs

Answer 11

Atypical T-Lymphocytes

Question 12

Forssman uses __________ that are injected into _________

Answer 12

Guinea pig cells, rabbit cells

Question 13

What are the antibodies formed in Forssman?

Answer 13

Anti-guinea pig cells and anti-sheep cells

Question 14

It is used for any substance that stimulated the formation of sheep hemolysin (Anti-sheep cells)

Answer 14

Forssman Antigen

Question 15

Antibodies produced by unrelated species (Plant, animal, human) which can cross-react with the same antigen

Answer 15

Heterophil Antibodies

Question 16

What are the cells used to differentiate the Three experiments?

Answer 16

1. Sheep cells
2. Ox/Beef Cells
3. Horse Cells
4. Guinea Pig Cells

Question 17

Heterophil antibodies in IM reacts with what cells?

Answer 17

Reacts with sheep, ox, and horse cells and NOT guinea pig cells

Question 18

Heterophil antibodies in Forssman reacts with what cells?

Answer 18

Reacts with guinea pig, horse, sheep cells and NOT in ox/beef cells

Question 19

Heterophil antibodies in serum sickness reacts with what cells?

Answer 19

Reacts with all four cells (Sheep, ox, horse and
Guinea pig ells)

Question 20

What cells are the most useful for differentiation of the THREE?

Answer 20

Guinea pig and Ox Cells

Question 21

In serum sickness, ________ can be used as control to evaluate and validate the reagent used.

Answer 21

Antibodies

Question 22

What is the Principle of Paul Bennet Test?

Answer 22

Hemagglutination

Question 23

Presumptive test for the presence for
heterophile antibodies

Answer 23

Paul Bennet Test

Question 24

Detects the antibodies of infectious
mononucleosis, Forssman, and serum sickness

Answer 24

Paul Bennet Test

Question 25

Paul Bennet test can identify the specific nature of the heterophil antibody. True or False

Answer 25

False Cannot identify the specific nature of the heterophil antibody and Can only tell if heterophil antibodies are present

Question 26

Paul bennet test is incapable of determining specificity and is only indicative of the presence or absence of heterophil True or False

Answer 26

True

Question 27

Reagent Antigen in Paul Bennet Test

Answer 27

2% suspension of sheep rbcs

Question 28

Antibody used in Paul Bennet test

Answer 28

Heterophil Abs in patient’s serum

Question 29

(+) in Paul bennet test means

Answer 29

there is hemmagglutination and presence of heterophil antibodies

Question 30

Principle of Davidson Differential test

Answer 30

Absorption hemagglutination

Question 31

What are the Two steps in Davidson Differential Test?

Answer 31

A. Absorption B. Hemagglutination

Question 32

Exposure of test serum to both beef cells and guinea pig cell which causes absorption of either one or both of these antibodies

Answer 32

Absorption

Question 33

Antigen in Davidson Differential Test

Answer 33

guinea pig kidney and beef rbcs

Question 34

Antibodies in Davidson Differential Test

Answer 34

heterophil abs in patient’s serum

Question 35

Indicator cells in Davidson Differential Test

Answer 35

Sheep RBCs

Question 36

The “Absorbed Agglutinins” (precipitates) are removed by centrifugation and the resultant fluid (supernatant) are then tested with Sheep RBC

Answer 36

Hemagglutination

Question 37

If the tube in Davidson is positive for absorption -> Antibody titer will _________ -> Agglutination ___________

Answer 37

decrease, weakens

Question 38

If the tube in Davidson is negative for absorption -> antibody titer will _________ -> agglutination is ____________

Answer 38

increase, strong

Question 39

ABSORPTION PATTERN Hetero Abs Beef RBCS GPKC Forssman _______. ________ IM ________. ________ Serum sickness ________ ________

Answer 39

ABSORPTION PATTERN Hetero Abs Beef RBCS GPKC Forssman No Yes IM Yes no Serum Sickness. Yes Yes

Question 40

AGGLUTINATION PATTERN Hetero Abs. Beef RBCS GPKC Forssman. _________ ______ IM _________ ______ Serum Sickness _______. _____

Answer 40

AGGLUTINATION PATTERN Hetero Abs. Beef RBCS. GPKC Forssman ++++ + IM + ++++ Serum Sickness ++ ++

Question 41

Principle of Monospot

Answer 41

Absorption Hemagglutination

Question 42

Antigen in Monospot

Answer 42

Guinea pig kidney cells and Beef RBCS

Question 43

Antibody in Monospot

Answer 43

Heterophil Abs in patient’s serum

Question 44

Indicator cell in Monospot

Answer 44

Horse Cells

Question 45

When Papain is added to sheep cells in Monospot, the receptors for the Abs are specifically _____________.

Answer 45

Inactivated

Question 46

Used to differentiate a sheep rbc that are native and those that Papain Treated

Answer 46

Rapid Differential Slide Test Using Papain-treated Sheep RBCs

Question 47

Principle of Rapid Differential Slide Test Using Papain-treated Sheep RBCs

Answer 47

Hemagglutination

Question 48

Is there an agglutination in normal serum and IM serum when Native Sheep RBC is used?

Answer 48

Yes

Question 49

Is there an agglutination in Serum sickness and other heterophil abs when Native Sheep RBC is used?

Answer 49

Yes

Question 50

Is there an agglutination in Serum sickness and other heterophil abs when Papain-treated Sheep RBC is used?

Answer 50

yes

Question 51

Is there an agglutination in normal serum and IM serum when Papain-treated Sheep RBC is used?

Answer 51

None or weak agglutination