ILO WEEK 6 Flashcards

1
Q

atresia

A

degeneration of ovarian follicles which do not ovulate

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2
Q

infertility definition

A

1 year of unprotected vaginal intercourse in the absence of known causes of infertility in women of reproductive age

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3
Q

Lifestyle factors affecting infertility (common factors)

A

smoking -> both sexes but particularly on the males
alcohol
recreational drugs
STIs (if ever)
Driving (male driver for long time driving; scrotum temperature; chefs as well warm over)
Toxins, radiation
Tight garments (looser fitting clothes)
Weight -> severe underweight and obese may cause problems

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4
Q

Describe groups I, II and III in irregular menstrual cycle

A

Group I -> low levels of endogenous gonadotrophins and oestrogen -> can improve chances by lifestyle factors (BMI)

Group III -> only option is oocyte donation; generally after menopause, in some women quite early

Group II -> variety; hormone profile is in the normal range; often PCOS; suitable for ovulation induction
+BMI (loose weight)

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5
Q

Effect of chlamydia on fertility

A

big damage before you attend to treatment;
HYDROSALPHINX inflammed fallopian tube; often bilateral
Egg cannot pass

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6
Q

HSG (Hysterosalpingography)

HyCoSy

A

imaging; shape of uterus; shows if tubes are blocked
(contrast material)

HyCoSy -> same but uses ultrasound

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7
Q

IVF steps

A
  1. Ovarian stimulation hormone therapy
  2. Egg pick up (aspiration) hollow needle into vacuum pump
  3. Sperm preparation
  4. Egg fertilization
  5. Embryo development
  6. Embryo transfer with a cannula
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8
Q

Ovarian hyperstimulation

A

Need to predict patients response (basing on many factors)

Suppression of the natural cycle

FSH injections stimulate growth of follicles, more than one

Close monitoring of serum estradiol and follicular growth by ultrasound

Would be best to get 8-10 eggs

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9
Q

Intra-cytoplasmic sperm injection (ICHSI)

A

Single sperm injected into the oocyte; does not damage the egg; deposited near the nucleus; need to make sure the egg got out of the meiotic arrest

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10
Q

OHSS Ovarian Hyper-stimulation syndrome

A

Can occur in fertility treatment;
Due to administration of gonadotrophins; higher risk in PCOS, under 30s; multiple pregnancy
Can lead to increased risk of pre-eclampsia

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11
Q

List protected characteristics

A
  • Age
  • Disability
  • Gender reassignment
  • Marriage and civil partnership
  • Pregnancy and maternity
  • Race and ethnicity
  • Religion and belief
  • Sex
  • Sexual orientation
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12
Q

Describe the holy triad of reproductive physiology

A

Ovary,
fallopian tube,
Uterus;
MOST IMPORTANT

All other parts need to also be doing their job!!!(thyroid; thymus; Hypothalamus; Pineal; Pituitary; Adrenals; Pancreas)

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13
Q

How semen quality is defined

A

Checked through morphology
There are lots of abnormalities (not so many normal ones)

WHO guidelines for reference values

  • total sperm number
  • sperm concentration
  • progressive motility
  • sperm morphology ( normal %) (normal is 4)
  • vitality (live sperm ~58%)
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14
Q

Assisted reproductive techniques

A
  • IVF
  • Selective salpingography (block the damaged tube -> then the other has greater chance for being successful)
  • clipping/ salpingectomy (removal of a tube)
  • IUI -> intra uterine insemination
  • IVF
  • IVF with ICSI ( with own or donor gametes)
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15
Q

Fertilisation and barriers preventing polyspermy

A

Need to overcome physical barriers first -> travel down the right fallopian tube; overcome chemical conditions; be there in the right time; have enough enzymes; be first

  • Fertilisation in the fallopian tube (oocyte + sperm)
  • Sperm penetrates oocyte -> enzymes on sperms head into zona pellucida - Zygote formed -> Day 1; DNA mixes -> division starts
  • 16 cells cleavage -> does not get bigger!!!; compact
  • Morulla 16 cells -> day 4
  • Blastocyst formation (32 or more cells) Embryonic cells
  • Zona pellucida still present
  • Implants into growing uterine wall DAY 7
  • First it sheds zona pellucida
  • Implants

Sperm needs to penetrate the corona radiata with the acrosome to get to the receptor in the zone pellucida

Then when one sperm gets through it depolarises the membrane which deactivates all the other receptors (only one can get through)

The depolarisation causes the granular ells to release their contents onto the surface of the zone pellucida making the oocyte impermeable and non penetrable ( using Na+ and Ca2+)

Then the genetic materials can fuse

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16
Q

Describe the development of the testes

A

SRY gene -> development of Leydig cells (testosterone made also by DIHYDROREDUCTASE) and Sertoli cells (anti-mullerian hormone)

17
Q

Describe the anatomy and histology of the male reproductive tract

A

MALE REPRODUCTIVE ANATOMY

Seminal vesicles
5 cm long glands
between bladder fundus and rectum (rectovesicle pouch and the rectoprostatic fascia)
combine with vas deferens to form the ejaculatory duct -> drain into the prostatic urethra
honeycomb; lobuled structure with mucosa lines pseudostratified columnar epithelium; highly influenced by testosterone; growing taller with high levels
responsible for production of seminal secretions

Embryology: SEED (derived from mesonephric ducts)
Seminal glands
Ejaculatory ducts
Epididymis
Ductus (vas deferens)

Secretions included in late ejaculate fractions
Alkaline fluid -> neutralises the acidity of the male urethra and vagina in order to facilitate the survival of spermatozoa
Fructose -> provides an energty source for spermatozoa
Prostaglandins -> have a role in suppressing the female immune response to foreign semen
Clotting factors -> designed to keep semen in the female reproductive tract post-ejaculation
Remaining volume is made up of testicular spermatozoa, prostatic secretions and mucus from the bulbourethral gland
Neurovasculatore
internal iliac artery: internal pudendal and middle rectal arteries
sympathetic
external and internal iliac lymph nodes

Bulbourethral glands
Cowper’s glands
Pea shaped exocrine glands located posterolateral to the membranous urethra superiorly to the bulb of the penis
producing a lubricating mucus secretion (containing glycoproteins
deep perineal pouch
open into the proximal portion of the spongy urethra
compound tubulo-alveolar glands lined by columnar epithelium
development greatly influenced by DHT (dihydrotestosterone) (derived from urogenital sinus)

The mucus
lubrincation
pre-ejaculate; prepares a clean and lubricated pathway for ejaculation; usually devoid of any spermatozoa (sometimes present)
Vasculature and innervation
arteries to the bulb of penis
hypogastric nerve, pelvic nerve and pelvic branch of pudendal nerve
internal and external iliac lymph nodes

Prostate
largest accessory gland in the male reproductive system
inferior to the neck of the bladder and superiorly to the external urethral sphincter (levator ani muscle lying inferolaterally)
prostatic glands take the proteolytic enzymes secrete them immediately before ejaculation (10-12 openings on each side)
size of a walnut
two-thirds of the prostate is glandular in structure and the remaining third is fibromuscular
surrounded by fibrous capsule of the prostate; not real capsule
3 zones:
central zone -> surrounds ejaculatory ducts, comprising approx. 25% of normal prostate volume (empty obliquely in the prostatic urethra)
Transitional zone -> located centrally; surrounds urethra, 5-10% of prostate vol; glands typically undergo benign hyperplasia (BPH)
Peripheral zone -> main body of the gland; high incidence of acute and chronic inflammation; high rate of prostate carcinoma at the peripheral zone; felt agains the rectum on DRE
fibromuscular stroma -> (fourth zone?) anterior; merges with tissue of the urogenital diaphram

neurovascular 
prostatic arteries; from internal iliac arteries
prostatic venous plexus
inferior hypogastric plexus
neurovascular bundles

The spermatic cord
begins in inferior abdomen and ending in the scrotum
formed at the opening of the inguinal canal (deep inguinal ring); laterally to the inferior epigastric vesels
cord passes through the inguinal canal entering the scrotum via the superficial inguinal canal

Fascial covering
External spermatic fascia -> derived from deep subcutaneus fascia (fascia innominata)
Cremaster muscle and fascia -> derived from the internal oblique muscle and its fascial coverings; forms the middle layer of the spermatic cord fascia. It is adiscontinuous layer of striated muscle that is orientated longitudinally
Internal spermatic fascia -> derived from the transversalis fascia

contents:
blood vessels (testicular artery
cremasteric artery and vein
artery to vas deferens
pampiform plexus of testicular veins
Nerves: genital branch of genitofemoral nerve; autonomic nerves
Other: Vas deferens (sperm from epididymis to the ampulla); Processus vaginalis (projection of peritoneum that forms the pathway of descent for the testes during embryonic development (in adults closed shut))
lymph vessels -> drain into the para-aortic nodes, lumbar region

Pampiform plexus: network of veins; venous drainage of testes; wrapping itself around testicular artery
acts as heat exchanger; cooling arterial blood
right drains into IVC; left into left renal vein
Vas Deferens
straight, thick muscular tube that conveys sperm from the epididymis to the ampulla ( then to ejeculatory duct)
Lots of smooth mauscle arranged in 3 layers (longitudinal, circular, longitudinal

The scrotum
fibromuscular cutaneous sac; between penis and anus
derived from paired genital swellings; biologically homologous to the labia majora
Contents:
testis -> site of sperm production
Epididymis -> situated at the head of each testicle; functions as a storage reservoir for sperm
Spermatic cord -. a collection of muscle fibres, vessels, nerves and ducts that run to and from the testes
+ muscle fibres; dartos muscle (sheet of smooth muscle; help regulate the temperature of the scrotum, wrinkling skin; decreases surface area, reducing heat loss

neurovascular supply
vessles: anterior and posteriol scrotal arteries
scrotal veins
anterior and anterolateral aspect of scrotal nerves; posterior aspect (posterior scrotal nerves derived from the perineal branches of pudendal nerve and posterior femoral cutaneous nerve
lymphatics -> superficial inguinal nodes

The testes and epididymis
paired structures within the scrotum; epididymis situated on the posterolateral aspect of each testicle
left testicle lower than the right; suspended from the abdomen by the spermatic cord
Originally located on the posterior abdominal wall; during embryonic development they descend down the abdomen through the inguinal canal to reach scrotum; they carry their neurovascular and lymphatic supply with them
sperm production and hormone synthesis; epididymis acts as storage
testes have an ellipsoid shape; series of lobules; each containing seminiferous tubules supported by interstitial tissue; lined by Sertoli cells (aid maturation process of spermatozoa)
Leydig cells responsible for testosterone production
Developing sperm travels through the tubules collecting in the rete testes; efferent tubules transport the sperm from the rete testes to the epididymis for storage and maturation.

inside scrotum, testes covered almost entirely by tunica vaginalis (closed sac of parietal peritoneal origin that contains a small amount of viscous fluid) it lubricates the surfaces of the testes and allowing for friction-free movement
testcular perenchymaprotected by tunica albuginea (a fibrous capsule the encloses testes, divides it into tubules)

Epididymis
Head -> most proximal part; formed by efferent tubules of the testes, which transport sperm from the testes to the epididymis
Body -> formed by the heavily coiled duct of the epididymis
Tail -> most distal part; marks the origin of the vas deferens, transport sperm to the prostatic portion of the urethra for ejaculation

Innervation -> testicular plexus; autonomic and sensory fibres
vascular supply -> testicular arteries; arise from the abdominal aorta (via inguinal canal)
cremasteric artery and artery of the vas deferens; give anastamoses to the main testicular artery
paired testicular veins; from pampiniform plexus
lymphatics -> lumbar and para-aortic nodes

The penis
sexual intercourse -> during erotic stimulation; undergoes erection; engorged with blood; ejaculation occurs; undergoes remission returning to a flaccid state
micturition -> penis important urinary role

Root -> most proximal, fixed part; located in the superficial perineal pouch of pelvic floor; not visible externally; contains tree erectile tissues (two crura and bulb) two muscles (ischiocavernosus and bulbospongiosus)
Body -> free part of the penis, between the root and glans; suspended from the pubic symphysis; composed of tree cylinders of erectile tissue: two corpora cavernosa and the corpus spongiosum
Glans -> most distal part; conical in shape; distal expansion of the corpus spongiosum; opening of urethra, termed the external urethral orifice

Erectile tissues
erectile tissues fill with blood during sexual arousal producing erection
left and right crura and the bulb of the penis; located laterally -> later form corpora cavernosa; separated by the spetum of the penis; often incompletely
bulb situated in the midline of penile root transversed by the urethra; later forms corpus spongiosum (ventral); male urethra runs through corpus spongiosum to prevent it becoming occluded during erection the corpus spongiosum fills to a reduced pressure

Muscles
bulbospongiosus (X2) -> associated with the bulb of penis; contracts to empty the spongy urethra of any residual semen and urine; anterior fibres aid in maintaining erection by increasing the pressure in the bulb of the penis
Ischiocavernosus (X2) -> surrounds the left and right cura of the penis; contracts to force blood from the cavernous spaces in the crura into the corpus cavernosa - helps maintain erection
Fascia coverings
two fascial coverings; most superficial layer, immediately under the skin -> external fascia of Colles (continuity with the fascia of Scapa which covers the abdominal wall)
deep fascia of the epnis (Buck’s fascia) continuation of the deep perineal fascia; strong membranous covering which holds all three erectile tissues together.
Ligaments
Suspensory ligament– a condensation of deep fascia. It connects the erectile bodies of the penis to the pubic symphysis.
Fundiform ligament– a condensation of abdominal subcutaneous tissue. It runs down from the linea alba, surrounding the penis like a sling, and attaching to the pubic symphysis.

Skin
more heavily pigmented than rest of the body; connected to the underlying fascias by loose connective tissue
prepuce (foreskin) is a double layer of skin and fascia; located at the neck of the glans; covers the glans to a variable extent; connected to the surface of the glands by the frenulum; a median fold of skin on the ventral surface of the penis; the potential space between the glans and prepuce is termed the preputial sac
Neurovascular supply
dorsal arteries of the penis
Deep arteries of the penis
Bulbourethral artery
All branches of the internal pudendal artery
Drained by paired veins; cavernous spaces are drained by the deep dorsal vein of the penis -> empties into the prostatic venous plexus; superficial dorsal veins
Innervation -> S2-S4 segments and spinal ganglia
pudendal nerve
Periprostatic nerve plexus

18
Q

Describe the coverings of the spermatic cord

A
  • Skin, Dartos & Colles
  • External Spermatic fascia
  • Cremaster muscle
  • Internal spermatic fascia
  • Tunica vaginalis (parietal)
  • Tunica vaginalis (visceral)
19
Q

Understand the anatomy of the inguinal canal and the path and control of testicular descent

A

Anatomy:
2 M, 2 A, 2 L, 2 T

Upper wall: 2 muscles

  • internal oblique muscle
  • transverse abdominus muscle

Anterior wall: 2 aponeuroses

  • aponeurosis of external oblique
  • aponeurosis of internal oblique

Lower wall: 2 ligaments

  • inguinal ligament
  • lacunar ligament

Posterior wall: 2 Ts

  • Trasversali fascia
  • conjoint tendon
PATH AND CONTROL OF TESTICULAR DESCENT
Trans-abdominal phase:
Passive; Weeks 10-15
gubernaculum; lateral to medial
control of testis INSL1

Trans-inguinal phase:
active; takes a couple of days (weeks 25-35)
processus vaginalis pushes its way through the tissue down, forming deep inguinal ring; takes various tissues with it;
testes with ductus deferent and testicular vessels follows
testosterone dependent

20
Q

Describe cryptochidism

A

Cryptochidism:
testies not descended; either bilateral ( more serious; cannot establish sex) or unilateral

When testes are too high -> too hot -> damage

If still undescended after 6 months -> operation!!! (before 1st birthday)
Hormonal treatment may also be offered but doesn’t always work

21
Q

Hypospadias

A

Hypospadias:
most common anomaly of the penis
ectopicallly positioned urethral meatus lies proximal to the normal site and on the ventral aspect; in severe cases opens on to the scrotum

Associated with cryptorchidism and inguinal hernia; maybe caused by hormonal fluctuations; advanced maternal age; IVF; teratogenic drugs or reduced sensitivity to androgens

treatment includes surgery and hormonal treatment
multiple procedures may be required

22
Q

Herniation

A

In situation when anterior abdominal wall develops a weakness; pressure can cause some of the bowel to be pushed out; the wall looses its integrity

usually keeps it all in place; cannot withstand the pressure if any damage is present

23
Q

Describe the common disorders and management of sex development

A

Any congenital conndition in which development of chromosomal, gonadal or anatomic sec is atypical;
Rare but crucial to get right

Congenital adrenal hyperplasia -> no cortisol and no aldosterone

5 alpha reductase deficiency -> no dihydrotestosterone

All newborns should receive a male or female sex assignment; strict confidence; open communication;

surgical management; Sex steroid replacement; Psychological management