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PHA338 > III. Biotechnology and Biopharmaceutical preparations > Flashcards

III. Biotechnology and Biopharmaceutical preparations Flashcards

1
Q

Pharmaceutical product manufactured by biotechnology methods from biological sources is defined as:

A. Biopharmaceutics
B. Bioavailable
C. Biopharmaceutical
D. Biotechnology

A

C. Biopharmaceutical

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2
Q

Less than 40 amino acids are:

A. Peptide
B. Protein
C. DNA
D. MRNA

A

A. Peptide

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3
Q

What covalent bond is involved in protein structure?

A. Disulfide bridge
B. Hydrogen bond
C. Salt bridge
D. Hydrophobic interaction

A

A. Disulfide bridge

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4
Q

The point at which the overall charge of the protein is zero.

A. pK
B. pI
C. pH
D. pU

A

B. pI

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5
Q

Which of the following are negatively charged amino acids?

A. Lysine (Lys, K), arginine (Arg, R) and histidine (His,H)
B. Alanine (ala), and Asparagine (asn)
C. Aspartic acid (Asp,D) and glutamic acid (Glu, E)
D. Cysteine (cys), Isoleucine (ile), and leucine (leu)

A

C. Aspartic acid (Asp,D) and glutamic acid (Glu, E)

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6
Q

What is a major problem with antisense drug?

A. High adsorption
B. Low biovailability
C. High cost
D. Low specificity

A

B. Low biovailability

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7
Q

Which is an antisense drug is available in the market?

A. Fomivirsen (Vitravene)
B. Epoetin Alfa (Epogen)
C. RecombivaxHB
D. Bevacizumab (Avastin)

A

A. Fomivirsen (Vitravene)

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8
Q

In polymerase chain reaction, at what temperature the desaturation happens?

A. 54 C
B. 72 C
C. 94 C
D. 198 C

A

C. 94 C

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9
Q

Protein aggregation can lead to:

A. Reduced bio activity
B. Blockage of pumps or tubings
C. Increased immunigenicity
D. All the other choices

A

D. All the other choices

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10
Q

Correct principal of life:

A. Protein to DNA to RNA
B. RNA to DNA to Protein
C. DNA to RNA to Protein
D. RNA to Protein to DNA

A

C. DNA to RNA to Protein

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11
Q

Which is a first generation monoclonal antibody?

A. Daclizumab
B. Adalimumab
C. Infliximab
D. Bevacomab

A

D. Bevacomab

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12
Q

Which two cells are used in hybridoma technology to produce monoclonal antibody?

A. Stem cell and myeloma
B. Macrophage cell and M Cell
C. Spleen cell and myeloma cell
D. T cell and B cell

A

C. Spleen cell and myeloma cell

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13
Q 
In gene therapy, the genetic materials are inserted into the body by using?

A. Monoclonal antibody
B. Recombination DNA
C. Retrovirus
D. Myeloma cell
A

C. Retrovirus

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14
Q

Which technology is used to produce monoclonal antibody?

A. Antisense technology
B. Recombinant technology
C. Hybridomatechnology
D. Polymerase technology

A

C. Hybridomatechnology

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15
Q

What protein’s behavior determines protein’s adsorption?

A. Number of amino acids, less than 40 amino acids shows more adsorption.
B. pH and pK of solvent and protein.
C. Isoelectric point pI, protein adsorption is very less near pI
D. Unfolding tendency, hydrophobicity & polyelectrolyte behavior of protein.

A

D. Unfolding tendency, hydrophobicity & polyelectrolyte behavior of protein.

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16
Q

The point at which the overall charge of the protein is zero (a neutral charge) is:

A. Isoelectric point
B. Isotonic point
C. Isolation point
D. Isobaric point

A

A. Isoelectric point

17
Q

One of the hypothesis of mechanism of antisense drug is:

A. Destroying the protein by macrophage cells
B. Inhibiting function of RNA by Ben onion chloride
C. By blocking the activation site of translation to protein synthesis.
D. Destroying RNA by strong acid.

A

C. By blocking the activation site of translation to protein synthesis.

18
Q

Product of combining two different DNA molecules using nucleus Ed such as restriction endonucleases is defined as:

A. Monoclonal DNA
B. Retro DNA
C. Messenger DNA
D. Recombinant DNA

A

D. Recombinant DNA

19
Q

What medium is used to culture the hybridoma cells to grow monoclonal antibody?

A. The culture is grown in HAT (hypoxanthine-aminopterin-thymine) medium
B. Hypoxanthine-guanine phosphoribosyl transferase (HGPRT) medium
C. Isotonic 0.9% NaCl and 5% Glucose medium
D. Strong acidic medium

A

A. The culture is grown in HAT (hypoxanthine-aminopterin-thymine) medium

20
Q

In DNA polymerase chain reaction, the denaturation process which occurs at 94 degree Celsius is defined as:

A. DNA is denatured to separate the two strands
B. Hybridizing a Nucleic acid primer to each DNA strand
C. DNA polymerase enzyme is added for extension of the primer
D. Inserting DNA into body using a retrovirus which is called vector.

A

A. DNA is denatured to separate the two strands

PHA338 (5 decks)

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