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Neurodegeneration > IHC - Abeta > Flashcards

IHC - Abeta Flashcards

1
Q

protocol

A
  1. deparaffinization and re-hydration
  2. antigen retrieval
  3. PAP circle
  4. washing (TBS)
  5. blocking (H2O2)
  6. washing (TBS-T)
  7. 1st Ab - human anti-20C2
  8. incubation (humid, 4C, 24h)
  9. washing (TBS-T)
  10. 2nd Ab - biotinylated goat anti-human
  11. washing (TBS-T)
  12. ABC
  13. incubation (2h)
  14. washing (TBS, Tris)
  15. DAB
  16. washing (TBS)
  17. dehydration
  18. coverslip (entellan glue)
  19. microscopy
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Neurodegeneration (8 decks)

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