IDT/ HLA Flashcards
Hepatitis B
Serology Window
60 days
Hepatitis B
NAT Window
20 Days
Hepatitis C
Serology Window
70 Days
Hepatitis C
NAT Window
7 Days
HIV
Serology Window
23 days
HIV
NAT Window
7 Days
Hep B Surface Antibody (sAb) +
Vaccinated
transfused from a immunized donor
Heb B
Surface Antigen (sAg) +
Core (cAb) +
Active Infection
Hep B
Surface Antibody (sAb) +
Core (cAb) +
Resolved
HBsAg
Hep B Surface Antigen
+ = infection
HBcAb
Hep B Core Antibody
Active or resolved disease
may be only marker of early infection
Not influenced by exposure to vaccine
Mandatory Infectious Disease Testing
a. HIV antibody (anti-HIV) donor screening test or HIV antigen/antibody (Ag/Ab) combination test
b. Hepatitis B surface antigen (HBsAg)
c. Hepatitis B core antibody (anti-HBc)
d. Hepatitis C antibody donor screening test (anti-HCV)
e. Hepatitis C ribonucleic acid (RNA) by donor screening or diagnostic nucleic acid test (NAT)
f. Cytomegalovirus (CMV) antibody (anti-CMV)
g. Epstein-Barr Virus (EBV) antibody (anti-EBV)
h. Syphilis donor screening
i. Toxoplasma Immunoglobulin G (IgG) antibody test
When would Transplant center is requesting pre-surgical nodes for tissue typing
your donor received a lot of blood products and steroid treatments
If 6 antigen mismatches are identified on your donor it means?
less likely chance of rejection; less likely chance of finding a recipient; Allocation will occur to national patients last
Total blood volume calc
donor’s weight in Kg divided by 0.015.
Round to the nearest tenth.
Total Plasma Volume calc
donor’s weight in Kg divided by 0.025.
Round to the nearest tenth.
Hemodilution- Total Infused
calculated amount of all infusion given to the patient by adding all blood and colloids given within
48 hours prior to blood draw
Round to the nearest tenth
Hemodilution- What period of time do you need to account for with blood and colloids
48 hours prior to blood draw
Hemodilution- Plasma Infusions
calculated amount of colloids given within 48 hours prior to blood draw
and adding all crystalloids given 1 hour prior to blood draw
Hemodilution- What period of time do you need to account for with Crystalloid
1 hour prior to blood draw
Blood Products
PRBC
Whole Blood
Reconstituted Blood
Colloids
Fresh Frozen Plasma (FFP), Platelets, Cryoprecipitates
Plasmanate, Plasma Protein Fraction,
Albumin, Dextran, Hespan, Hetastarch, Plasbumin, Volven
Crystalloids
Medications, Normal Saline (NS), Lactated Ringers (LR), Dextrose Solutions (D5W) and/or Hyperalimentation Fluids (TPN)
If Donor is <28 days old what also must be done?
sample MUST be obtained from the birth mother
HBsAb+
Vaccinated
What Heb B sero would be + if vaccinated
HBsAb ONLY
HBsAg
current hepatitis B infection.
HBsAb+
HBcAb+
RESOLVED INFECTION
infected or exposed to hepatitis B virus in the past, cleared the virus, and are now immune against another infection with hepatitis B.
HBcAb+
once were infected with hepatitis B
Candidates with high PRA indicates what?
high likely-hood of a positive crossmatch
how many antigens are used to determine tissue typing / crossmatches
6
what does a positive crossmatch indicate
organ is unacceptable for candidate
