ICPP session 1-5

Question 1

Major drug targets

Answer 1

RITE- receptors, ion channels, transporters, enzymes

Question 2

4 Receptor types

Answer 2

K(L)ING- kinase linked, (ligand gated) ion channels,nuclear/intracellular, G protein coupled

Question 3

Typical inorganic ion concentrations

Answer 3

ION INTERSTITIAL WATER INTRACELLULAR CONC.
Na+ 145mM 12mM
K+ 4.5mM 140mM
Cl- 123mM 4mM
Ca2+ 1.5mM 10-7 M

Question 4

Cell resting potentials

Answer 4

Cardiac muscle myocytes: -80mV
Neurones: -70mV
Skeletal muscle myocytes: -90mV
Smooth muscle myocytes: -50mV

Question 5

Action potential relative sizes and duration in different tissues

Answer 5

Axon: 0.5ms, +100mV
Skeletal muscle: 0.5ms, +130mV
Sino-atrial node: 100ms, +90mV
Cardiac ventricle: 100ms, +120mV

Question 6

SRP mechanism

Answer 6

Cytosolic+membrane proteins synthesised by ribosomes, when SRP recognises leading sequence @ N terminal end protein synthesis is halted, SRP is recognised by signal sequence receptor on ER membrane (part of protein translocator complex), stop transfer signal exists on membrane proteins causing protein to be embedded in ER membrane

Question 7

Erythrocyte cytoskeleton

Answer 7

Spectrin+actin anchored to erythrocyte membrane via adapter proteins called ankyrin+band 4.1 to integral membrane proteins band 3 and glycophorin A