IA Chapter 1 Origin Of Life Flashcards

1
Q

How do we define life?

A

Most biologists would identify two key features that indicate life:

  • the capacity for self replication
  • the capacity to undergo Darwinian evolution
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2
Q

Why is carbon the perfect molecule for life?

A
  • Carbon can form chemical bonds with many other atoms including H, N, O, P, S, Fe, Mg, Zn.
  • Carbon can form compounds that readily dissolve in liquid water.
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3
Q

Why is water essential for life?

A

Water allows molecules to dissolve and chemical reactions to take place.

Water exists as a liquid at a temperature range that is not too cold to sustain biochemical reactions and not too hat to prevent many organic bonds from forming.

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4
Q

Why is water such a good solvent?

A

Water is a great solvent due to the strong intermolecular forces of attraction caused by the hydrogen - oxygen bond. Water molecules are extremely polar.

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5
Q

What are hydrophilic molecules?

A

These are polar molecules and therefore have a high affinity for water. This means they are soluble.

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6
Q

What are hydrophobic molecules?

A

These are apolar molecules (not polar) which means that they are relatively insoluble.

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7
Q

What are lipids? (fats and oils)

A

These diverse molecules consist of a hydrophilic head and tails. These are poorly soluble in water. Lipids are a convenient way to store chemical energy and their weak bonding within their macromolecular results in a high degree of flexibility that is useful in membranes (phospholipid bilayer).

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8
Q

What are carbohydrates?

A

These are molecules that have many hydroxyl groups (-OH) attached. These hydroxyl groups are polar, making carbohydrates soluble.

Sugars an a common form of carbohydrate that typically contain a ring of 5/6 carbons ( pentose and hexose respectively)

Monomers can chain together via polymerisation to form polysaccharides which are useful energy stores and can also provide structural support for organisms.

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9
Q

What is polymerization?

A

This is the process where individual monomers are joined together to form a polymer.

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10
Q

What are proteins?

A

Proteins are made of chains of amino acids that are linked together to form chains.

There are 20 different amino acids found in living systems and the order of the amino acids in a chain determine the proteins function.

Proteins can provide structure or act as catalysts (enzymes).

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11
Q

What is a catalyst?

A

A catalyst is a substance which increases the rate of reaction by lowering the activation energy of the reaction whilst not being used up in the process.

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12
Q

What are **nucleic acids **?

A

There are the largest macromolecules and can be found as single units called nucleotides or linked together to form longer polymers.

Each nucleotide contains:
- a five carbon sugar molecule
-one or more phosphate groups (PO4 3-)
- a nitrogen containing compound called a nitrogenous base

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13
Q

What are the 4 bases found in DNA?

A
  • Adenine
    -guanine
  • cytosine
  • thymine

In the double helix adenine and thymine are always a pair and guanine and cytosine are always a pair.

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14
Q

What is DNA (deoxyribonucleic acid)?

A

DNA consists of two long molecular strands coiled about eachother to form a double helix. Bonds that resemble the steps of a spiral staircase connect the two helical strands. The steps consist of two nucleotides which are held together by weak hydrogen forces.

The bases always match A-T and C-G.

The bases are attached to their helical strands by sugar groups which in turn are connected together along the exterior of the helix by phosphate groups.

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15
Q

How does DNA replicate?

A

Special proteins unzip strands of DNA. The single strands hook up with spare nucleotides in the legend around the molecule. Each base in the unzipped strand latches onto its complementary base and the sugar and phosphate groups of the newly acquired nucleotides join together into helical strands.

Two identical double helix molecules are formed exactly like the original.

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16
Q

What is DNA hybridisation?

A

DNA double helixes can be separated into two separate strands using heat and mixed with the DNA of another species. If the DNA from the two separate species joins, you can reheat it to see at what temperature the DNA splits. The higher the temperature the new DNA splits the more closely related the species are, and the more the nucleotide pairs match.

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17
Q

What is genetic code?

A

The genetic code is a set of ‘instructions’ in the form of a specific sequence of nucleotides contained in DNA. It is crucial for protein synthesis.

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18
Q

What is RNA?

A

RNA is very similar to DNA, but is used to transcribe the DNA so that it can be replicated.

The difference between the two is that RNA contains the sugar ribose as opposed to deoxyribose. In RNA the base thymine is swapped for uracil.

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19
Q

What is transcription? (mRNA)

A

A DNA double helix is unzipped. Once split the DNA nucleotides seek out complementary RNA nucleotides to produce a strand of mRNA. The mRNA is then released and the DNA helix zips together again. The released mRNA carries its version of the DNA sequence to a region that has free amino acids where molecular factories called ribosomes use the mRNA to combine amino acids into long protein chains.

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20
Q

What are the basic constituents of a cell?

A

Nucleus - Center of a cell where genetic information is stored.
Cytosol - a saltwater solution containing enzymes and ribosomes.
Cell membrane - Surrounds the cell made of a phospholipid bilayer.
Cell wall - provides the cell with protection and rigidity, made of carbohydrates and short-chain amino acids.

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21
Q

What is the function of a cell?

A

It provides an environment for biochemical processes to take place and genetic information to be stored.

It provides the basic structure for all life.

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22
Q

What is a biomarker?

A

This is a term that is used for any evidence that indicates present or past life, either in situ or remotely.

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23
Q

What are the six categories of biomarkers?

A

1 - cellular remains.
2 - textural fabrics in sediments that record the structure and/or function of biological communities.
3 - biologically produced (biogenic) organic matter.
4 - minerals whose deposition has been affected by biological processes.
5 - stable isotopic patterns that reflect biological activities.
6 - Atmospheric constituents whose relative concentrations require a biological source.

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24
Q

What are the two approaches to the origins of life?

A

1 - a ‘bottom-up’ strategy
2 - a ‘top-down’ strategy

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25
What is the top-down strategy for the origins of life on Earth?
This strategy looks at present-day biology and extrapolates backwards towards the simplest living entities.
26
What is the bottom-up strategy for the origins of life on Earth?
This approach focuses on a collection of inanimate elements, molecules and minerals from the past with known properties and attempts to figure out how they may have become combined to create a living organism.
27
What are **aromatic hydrocarbons**?
These are organic molecules that consist of a six-carbon ring held together by alternating single and double-carbon bonds.
28
Where can organic molecules be produced?
Organic molecules can be produced in candle flames, from burning coal, from stars and in interstellar molecular clouds.
29
How are organic molecules produced in molecular clouds?
The temperatures are so low in molecular clouds that any element heavier than H or He will stick to the surface of any dust grains it comes into contact with. Chemical reactions between organic compounds are catalyzed by the grain surface. Then they are further processed by UV and cosmic rays. The products of grain mantle chemistry may take part in further organic chemistry when they are incorporated into the warm (200/400 K) and dense areas of gas around recently formed stars.
30
What are **hot cores**?
These are the hot dense molecular clouds around newly formed stars which are the most-chemically diverse regions of the universe. Various interesting chemical compounds are produced when icy grain mantles are heated in hot cores. Radiation evaporates the ice and organic compound return to a gaseous state.
31
What do hot cores develop into?
Hot cores become **solar nebula** which eventually become a new solar system containing all the new organic molecules.
32
What were the two roles of **energy** in the origin of life?
It could have felled chemical reactions that synthasised organic matter in the early solar system and sustained primitive life.
33
What were the early sources of radiation on the earth?
- Solar radiation would have been the most prominent - geothermal heat flow - electrical discharges (lightning) - cosmic rays - shockwaves (atmospheric waves)
34
What was millers origin of life experiment?
Millers experiment replicated the oceans and atmosphere of the early earth. He boiled the solution and subjected the 'atmosphereʼ to a continuous electric current. After a week his experiment showed that organic compounds, specifically amino acids were easily produced and were likely very abundant in the universe. This experiment showed organic compounds could be produced in the hydrosphere of a planet.
35
What is significant about the **murchison meteorite**?
The murchison meteorite feel in Australia in 1969.it was a rare type of **carbonaceous chondrite** that was found to contain many organic molecules, including amino acids. The amino acids found in the meteorite are both similar in type and abundance to those formed in the miller Urey experiment.
36
Why would the results from the miller Urey experiment be hard to apply to earths early atmosphere?
The miller Urey experiment relys on a reducing atmosphere, however methane and ammonia both break down in the presence of UV radiation. Therefore it is believed that earths early atmosphere consisted of carbon dioxide, nitrogen and water which were less favourable for producing amino acid in the experiment.
37
How can organic molecules be created through impacts?
- Shock waves from impacts may have forced gas to combine, producing organic matter. - impactors may vaporise rock on impact allowing organic molecules to form as gasses recombine.
38
What is **chirality**?
This refers to how some molecules can have an identical chemical structure, but differ in their structural arrangements. These molecules are known as **isomers**. **Chiral** molecules are molecules that when reflected, could not be superimposed on its mirror image. **Achiral** molecules are molecules that would be superimposeable on their mirror image.
39
How to identify a **chiral molecule**?
They are usually carbon bonded to 4 different groups.
40
How to name "left" and "right" handed chiral molecules?
- Right landed forms are abbreviated to D (dextro). - left handed forms are abbreviated to L (levo).
41
What is a **racemic mixture**?
This is a mixture that has an equal number of left and right handed molecules. These mixtures are typically created in the absence of life.
42
What chirality do all amino acids used in protein have?
All protein can only be made from left handed amino acids apart from glycine which is achiral. A mix of left and right handed amino acids in protein would hinder the proteins from performing their biological functions.
43
What is **ultra violet circularly polarised light (UVCPL)**?
This is a type of starlight where in electric field direction rotates along the beam. This rotation con occur in a left or right handed direction. Chiral molecules have different absorption intensities for left and right UVCPL, therefore UVCPL destroys one form of the molecules more readily than the other.
44
What type of molecule in respect to their chirality are most commonly found in meteorites and why?
Meteorites contain mainly left handed molecules. This could be due to: -UVCPL destroying right handed variety's. - the chirality may have been inherited by molecules that grew on certain surfaces such as magnetite.
45
What is **panspermia**?
This is a theory that suggests that microbes can be carried between interstellar bodies via comets and meteors.
46
What are 3 **concentration mechanisms**?
1. Marginal marine environments such as lagoons and tide pools. Evaporation increases concentrations in residual liquids. 2. Freezing an aqueous solution increases the concentration of any organic molecules as the water freezes first. 3. The surfaces of days and other minerals provide sites for trapping organic matter.
47
Why are **concentration processes** important?
They are important for providing enough localised raw material for the creation of primitive living systems.
48
How are **polymers** and **macromolecules** formed?
These are formed by either two -OH groups undergoing a condensation reaction, or a -NH2 group reacting with a -COOH group. These processes would allow single monomer sugars and amino acids to form larger organic molecules, although water is destructive to polymers.
49
What are **amphiphilic molecules** and how do they work?
They are single molecules that process both a **hydrophilic head** and **hydrophobic tails**. The hydrophilic head has a small charge, making it soluble in polar solvents, such as water. The hydrophobic tails have no charge making them insoluble. (phospholipids)
50
What are **micelles**?
If a solution containing amphiphilic molecules is shaken, the amphiphilic molecules group together and form spheres with the soluble heads outside and the insoluble tails inside called **micelles**.
51
What is a **bilayer vesicle**?
This is when there are enough anphiphilic molecules to form a bilayer with water on both the outside and inside. All the **biylayer vesicles** and **micelles** can form organically.
52
What are **coacervates**?
When proteins are dropped in water they group together into droplets called **coacervates**. Many substances when added to coacervate preparation can become incorporated into the coacervates. This is potentially how prebiotic chemical factories were made.
53
What is a ** microsphere**?
Amino acids were dry heated to cause polymerisation by condensation reaction. These polymers resembled proteins so were named **proteinoids**. These proteinoids were then introduced into hot water and allowed to cool. The proteinoids formed small spheres 2 microns in diameter that had a double wall resembling a biological membrane. These spheres could expand and contrast depending on the salt concentration of the water and were coined **microspheres**. When left microspheres absorbed more proteinoids over time.
54
Why were minerals important for early life?
- Small pockets in mineral may have provided protection from dispersion and destruction for early chemical systems. - mineral may have acted as catalysts for chemical processes. - minerals could have acted as support structures to support molecules and allow them to accumulate. - certain minerals such as calcite ham a mirrored structure so when bonding with amino side can produce protein like structures that are inherently left or right handed.
55
What came first: DNA, RNA or proteins?
RNA can store genetic information and can have some of the catalytic properties of enzymes therefore, may have predated DNA and proteins. Arguments to support this: - nucleotides in RNA are more easily synthesised than nucleotides in DNA. - its easy to imagine DNA evolving from RNA, given DNA 's greater stability. - RNA likely evolved before proteins as it is not believed that proteins can replicate in the absence of RNA.
56
What are **autotrophs**?
These are organisms that use photosynthesis or chemosynthesis (chemical energy) to generate energy and use for metabolic reactions.
57
What is **fermentation**?
This is a form of metabolism where the carbohydrate glucose (C6H12O6) is transformed into CO2 and ethanol (CH3CH2OH) or lactic acid (C3H6O3) for a net gain equivalent to that stored in two phosphate bonds.
58
What is **respiration**?
This is where glucose reacts with oxygen from the earths atmosphere to form carbon dioxide and water with a net gain equivalent to 36 phosphate bonds, far better than fermentation.
59
What are **heterotrophs**?
These are organisms that gain their energy from consuming other organisms such as autotrophs.
60
What is the probable common ancestor of all life on earth?
By comparing the ribosomal RNA of many organisms we can put together a tree that represents how closely related different organisms are.
61
What is a potential common ancestor for every living organism on earth as shown by **moleculer phylogeny**?
By comparing ribosomal RNA we can hypothesise that our last common ancestor may have been heat loving chemosynthetic organisms that populate hydrothermal vents today. This does not however suggest these were the first organisms on earth.