I. DNA Extraction

Question 1

what is DNA?

Answer 1

genetic material found in cells

Question 2

function #1 of DNA?

Answer 2

contains instructions for development, functioning, growth, and reproduction of living organisms

Question 3

function #2 of DNA?

Answer 3

codes info for protein synthesis, which determines biological traits and cellular functions

Question 4

function #3 of DNA?

Answer 4

transfers genetic traits from one generation to the next

Question 5

step #1 - item - function

Answer 5

1. blend fruits - blender / cell wall lysis buffer - breaks down cell walls

Question 6

step #2 - item - function

Answer 6

2. strain fruit juice - strainer - filters out solid debris

Question 7

step #3 - item - function

Answer 7

3. add detergent / lysis buffer - solubilizes membranes and proteins

Question 8

step #4 - item - function

Answer 8

4. add NaCl / wash buffer - creates environment for DNA precipitation

Question 9

step #5 - item - function

Answer 9

5. add meat tenderizer (papain) / protease enzyme - breaks down proteins (optional)

Question 10

step #6 - item - function

Answer 10

6. add ice cold alcohol / isopropanol - precipitates the DNA

Question 11

step #7

Answer 11

7. twirl precipitated DNA clumps with a toothpick

Question 12

step #8

Answer 12

visualisation with gel electrophoresis