Hydrogenases

Question 1

What are hydrogenases + what is their reaction

Answer 1

enzymes that catalyze the simplest reaction
2H + 2e <–> H2

Question 2

What does the direction of the reaction depend on?

Answer 2

The E of electron donor/acceptor

Question 3

Where is hydrogenase found?

Answer 3

archaea & bacteria

Question 4

What do the bimetallic active site consist of?

Answer 4

Co & [CN]-

Question 5

How does structure promote function?

Answer 5

highly string field ligands promote H2 binding & heterolytic cleavage

Question 6

What are the 3 generic forms of hydrogenase?

Answer 6

[NiFe]. [FeFe]. [Fe]

Question 7

What is the function of [NiFe]?

Answer 7

Used for H2 oxidation

Question 8

What does Scys do in [NiFe]?

Answer 8

H shuttle sites

Question 9

What is the function of [FeFe]?

Answer 9

Used for both H2 production & uptake

Question 10

Where is the et pathway to/from in [FeFe]?

Answer 10

to/from FeFe site via a number of FeS clusters

Question 11

What does (SCH2)2NH do in [FeFe]?

Answer 11

H shuttle site