Hybrid capture assay group presentation

Question 1

The hybrid capture assay was first introduced to HPV testing by

Answer 1

Digene corporation

Question 2

The second generation hybrid capture assay was launched in

Answer 2

1997 is known to yield faster results than the Branched DNA assay

Question 3

The founder of Digene Corporation is

Answer 3

Floyd taub and was instrumental in building one of the first molecular instruments that allowed for the analysis of mutations of cancer in DNA that was inexpensive, routine, and accurate.

Question 4

Floyd taub graduated from

and was the CEO of

Answer 4

Northwestern university and was the CEO of cytocore

Question 5

Floyd taub was the founder of

Answer 5

Dovetail technologies

Question 6

Interpretations

The detection of the substrate aka Alkaline phosphatase can be measured from a

Answer 6

Luminometer which is a photometer that can detect significantly low levels of light that is given off from the substrate

Question 7

Interpretations

The units that are used are

Answer 7

Relative light units and for example if a specific sequence of DNA is HPV positive then that would mean the relative light units is over 1.0 which equals 1 pg of HPV DNA per mL.

Question 8

Interpretation

Some benefits of using a luminometer are

Answer 8

great sensitivity, low cost, and low background interferences.

Question 9

Interpretation

The hybrid capture assays allows for the interpretation of not only HPV but of

Answer 9

Chlamydia, Gonorrhea, CMV, and Hepatitis B

Question 10

Calibration

The hybrid capture assay requires calibration with every

Answer 10

Run

Question 11

Calibration

Must have a positive and negative

Answer 11

Calibrator

Question 12

Calibration

Positive calibrator

Answer 12

Furthermore, a positive calibrator control must be used and have a coefficient of variation of 15% or less

Question 13

Calibration

Negative calibrator

Answer 13

negative control that is less than 250 RLU and have a coefficient of variation of less the 25%