Human Herpes Virus

Question 1

What is HHV5 otherwise known as and what disease does it cause?

Answer 1

Human cytomegalovirus, cytomegalic inclusion disease.

Question 2

Name three proteins in the tegument.

Answer 2

VP16, ICPO, ICP4

Question 3

How does the HSV genome replicate?

Answer 3

Via rolling circle to generate long progeny HSV DNA concatemers, which are cleaved and packaged into progeny viral particles.

Question 4

What are a) VP16 b) ICPO and ICP4 c) vhs?

Answer 4

a) alpha-TIF b) immediate early proteins c) virion host shut off

Question 5

What is ICPO?

Answer 5

A ubiquitin ligase with degrades host repressors of HSV DNA transcription.

Question 6

Outline lytic replication.

Answer 6

Tegument fragments enter the nucleus, VP16 attached to HCF-1 and this complex binds Oct-1, HSV DNA rearranges so that the complex can bind the TAATGARAT sequence. SETs on the HSV DNA are replaced with HATs and there is transcription of IE genes involving ICPO and host RNAPII.

Question 7

What are four HSV1 entry receptors? What are their roles?

Answer 7

gB (fusogen), gD (fusion trigger), gH + gL (fusion regulator)

Question 8

Name three HSV proteins against host metabolism.

Answer 8

ICPO, vhs, and ICP27

Question 9

Describe four interactions between HSV and its host.

Answer 9

Arrest in G1, interference of host signal transduction pathways, apoptosis repression prior to viral release, disruption of cytokines-mediated signally networks.

Question 10

Which classification of the HHV has a predilection for B-lymphocytes?

Answer 10

Gammaherpesvirus