How we see Flashcards
what wavelengths of light will our retina respond to?
Our retina will only respond to images of light rays between 400-800 nanometres
How many layers does the retina have?
10
Where do the rods/cones sit within the retina layers?
they sit pretty deep in the outermost layer called the outer segment
light has to travel through all the layers to hit them
what contains rods/cones
discs/lamellae contain photoreceptors (rods and cones)
what is each lamellae made up of?
a cell membrane
integrated into this membrane is the visual pigment (proteins) rhodopsin in rods and cone opsins
how many types of cones are there? and what are they called?
3
S, M and L
Short, medium (green) and long (red) wavelength types
What is the only difference between rods and cones?
they respond to different wavelengths of light
What is chromophore formed from?
dietary vitamin A
What happens when light hits rod?
11-cis retinal (vit A compound) changes from cis to trans - alters shape
what is rhodopsin made up of? (2)
opsin and 11-cis retinal (helical protein)
when vit A changes shape what happens to rhodopsin?
rhodopsin splits and bleaches as not all trans retinal can fit in the opsin
Bleaching of the visual pigment results in what?
phototransduction cascade
What role does Vitamin A play in the visual pigment?
visual pigment regeneration
To correct the cascade after 1 photon of light we need energy (ATP) – channel opens again and ready for business again. Need to get Vit A to convert back to normal shape and latch onto rhodopsin so that rhodopsin is ready for next photon of light
Describe the phototransduction cascade
When light hits vit A – changes from cis to trans
Vit A changing shape results in a cascade of reactions - these spread through until they meet an optic nerve cell
ultimately causes hyperpolarisation (Na+ channels close) and AP produced in optic nerve cell
Then pigment epithelial cells convert trans –retinol (vit A) back to cis-retinol this is then transported back to rod/cone to reform opsin
what is different about photoreceptor cells in comparison to other cells in the body
photoreceptor cells are, at rest (in the dark), kept in a depolarised state by open Na+/Ca+ channels (not closed like normal)
What are pigment epithelial cells and what is their function?
Cells in the deepest part of the retinal layer
All-trans retinol taken up here and converted back to cis retinol which can then go back to make rhodopsin
Want to absorb it so that it’s not bouncing back and making multiple images on the retina
What happens to vitamin A along the regeneration pathway
At certain points along the way – lose a bit of Vit A which can’t be reincorporated into the regeneration process so we need to keep replenishing it through our diet
E.g Fish oil, carrots
Vitamin A deficiency
any condition that affects vitamin A absorption will affect vision – (night) blindness.
Vitamin A is also essential for healthy epithelium. So conjunctiva and corneal epithelium are also abnormal.
can occur in conditions such as malnutrition, malabsorption syndromes such as coeliac disease, sprue (immune (allergic) reaction to gluten).