how to investigate effect of pH on the rate of an enzyme controlled reaction Flashcards
1
Q
1.
A
prepare a range of different pH solutions
2
Q
2.
A
mix each pH solution with enzyme e.g. amylase
3
Q
3.
A
prepare test tubes also of starch solution
4
Q
4.
A
equilibrate the tubes of pH solution of amylase and starch in a water bath to 37 degrees
5
Q
5.
A
mix together the pH amylase solution and starch
6
Q
6.
A
every 30 seconds remove a sample
7
Q
7.
A
place sample on a dimple tile containing a drop of iodine - note the colour change
8
Q
8.
A
continue until the iodine is no longer BLACK - showing that STARCH has been DIGESTED - record the time taken to DIGEST the STARCH
9
Q
9.
A
repeat for each pH and at least 3 x for each pH
10
Q
10.
A
calculate a mean time for each pH
11
Q
11.
A
control variables - temperature, concentration of starch, amylase
