how to investigate effect of pH on the rate of an enzyme controlled reaction Flashcards

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1
Q

1.

A

prepare a range of different pH solutions

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2
Q

2.

A

mix each pH solution with enzyme e.g. amylase

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3
Q

3.

A

prepare test tubes also of starch solution

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4
Q

4.

A

equilibrate the tubes of pH solution of amylase and starch in a water bath to 37 degrees

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5
Q

5.

A

mix together the pH amylase solution and starch

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6
Q

6.

A

every 30 seconds remove a sample

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7
Q

7.

A

place sample on a dimple tile containing a drop of iodine - note the colour change

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8
Q

8.

A

continue until the iodine is no longer BLACK - showing that STARCH has been DIGESTED - record the time taken to DIGEST the STARCH

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9
Q

9.

A

repeat for each pH and at least 3 x for each pH

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10
Q

10.

A

calculate a mean time for each pH

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11
Q

11.

A

control variables - temperature, concentration of starch, amylase

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