Homework - Nonfermenting/Fastidious Gram Negative Bacteria Flashcards
1
Q
Eikenella corrodens
A
- thin GNB
- pits agar
- require heme to grow (no growth on MacConkey)
- small gray to pale yellow
- bleach odor
- oxidase negative
- ornithine decarboxylase negative
- catalase negative
- can produce Beta lactamase
- dental infectino
- wounds / trauma
- human bites
- HACEK
2
Q
Capnocytophagia
A
- GNB
- pointed ends
- gliding motility (swarm(
- tiny pinpoint yellow-orange colonies
- Human: indole, oxi, catalase negative
- Animals: catalase and oxidase positive
- can produce B-lactamase
- dental infections
- endocarditis
- asplenic patients are at risk
3
Q
Cardiobacterium hominis
A
- GNB
- pleomorphic, swollen ends, forms rosette
- pale yellow to white colonies
- don’t grow on macconkey
- oxidase positive
- catalase negative
- can produce B lactamase
- endocarditis
4
Q
Sphingomonas
A
- long slender GNB
- motile by polar flagella
- culture 18 - 22 degrees
- strong yellow colony
- catalase +
- oxidase +
- indole -
- trypsin +
- widely distributed
- rarely can cause line infection
5
Q
Pseudomonas aeruginosa
A
- long, thin GNB
- grow at 42 degrees C
- mucoid strains (alginate material)
- catalase positive
- motile
- oxidase positive
- pigment producing: pyoveidin (yellow green), florescent, pyocyanin (blue-green)
- Beta hemolytic, flat, spreading, metallic, can be mucoid
- nosocomial infection
- swimmer’s ear
- endocarditis in IV abusers
- osteo with puncture wound
- cystic fibrosis
- in burn pt: respiratory infection
6
Q
Acinetobacter
A
- small GN coccobacilli
- single and pairs
- plump and can be gram variable
- 2nd most common nonfermenter after P. aeruginosa
- smooth, opaque, grey-white to yellow colony
- some are Beta-hemolytic
- variable on Mac (if grow > purple)
- wounds
- catheter site
- respiratory and urinary tract with use of equipment and long stay in ICU
7
Q
Nesseria gonorrhoeae
A
- GN diplococci
- kidney bean shape
- intracellular
- modified Thayer-Martin agar
- require chocolate media for growth
- oxidase positive
- catalase positive
- always a pathogen when isolated
- ocular infection in newborns
- usually isolated
- nucleic acid amplification techniques
- superoxal (super catalase)
- can grow slowly on blood agar
- don’t grow on mac
8
Q
Neisseria meningitidis
A
- GN diplococci
- grow on blood
- catalase positive
- oxidase positive
- utilizes glucose and maltose
- vaccine does not cover B
- colonizes oral and oropharynx (10-30%)
- isolated by blood/csf culture
- pathogen: colostin resistant
- non pathogen: colostin susceptible
9
Q
Sapprophytic Neisseria spp
A
- can be elongated
- utilize sugar of first letter in species name
- oxidase positive
- rarely can cause line infection
10
Q
Moraxella catarrahlis
A
- GN diplococci
- slightly larger
- hockey puck colony
- catalase positive
- oxidase positive
- indole negative
- trypsin negative
- DNAase positive
- otitis media, URI, eye, pneumonia
- Beta lactamase production
- no glucose utilization
11
Q
Moraxella spp
A
- small, plump, GN coccobacilli in pairs and short chains
- most don’t grow on MacConkey
- non motile
- penicillin disk ?
12
Q
Burkholderia cepacia complex and gladioli
A
- Straight or slighly curved GNB
- motile
- catalase positive
- oxidase positive (cepacia)
- oxidase negative (gladioli)
- smooth, round, opaque and ?
- pink on mac
- Colestin resistant
- very resistant to antibiotics
- opportunistic infection in CF
- nosocomial infections
- catheter associated UTI
13
Q
Stenotrophomonas maltophilia
A
- GNB
- round, opaque, smooth and non hemolytic with greenish hue
- ammonia oder
- grow on Mac
- oxidase negative
- resistant to many drugs
- sensitive to Bactrim and colestin
- DNAse
14
Q
Rhizobium radiobacter
A
- small - medium GNB
- round smooth, non-pigmented to buff colored
- pink on mac
- can be mucoid
- catalase positive
- indole negative
- trypsin positive
- urease negative
- motile
- oxidise many sugars
- contaminated catheters or implanted prosthetics
15
Q
Chryseobacterium/Elizabethkingia
A
- GNB
- thinner in center and can be filimentous
- can be hemolytic, mocoid, pigmented yellow
- catalase negative
- oxidase negative
- indole positive
- non-motile
- neonatal meningitis (menegioseptia)
