Histopathology and cytopathology Flashcards
What do histopathologists use?
Biopsies
Resection specimens- from surgery
Frozen sections- real time diagnosis
Post-mortems
What do cytopathologists use?
Smears
Fine needle aspirates
What are the questions that are asked when reviewing histopathology biopsies?
Is it normal?
Is it inflamed and if it is, what is the cause?
Is it cancer and if it is, what type is it?
What are the questions that are asked when reviewing resection specimens?
How far has the cancer spread?
Is it all out?
How are sections obtained?
Specimen must be properly labelled
Fix in formalin
Embed in paraffin wax
Cut sections
What do we do with the sections?
Stain e.g. gram, ZN
Identify specific antigens using antibodies = immunohistochemistry
Carry out molecular tests
How long does it take cytopathology and histopathology results to reach the clinician?
Cytopathology - pretty fast
Frozen section- 30 minutes
Biopsies- 2-3 days
Resection specimens 5-7 days