histopath

Question 1

Biopsy result

Answer 1

5-7 days

Question 2

Decalcifying agents

Answer 2

acids and chelating agents

Question 3

purpose of acid alcohol

Answer 3

decolorizer

Question 4

tissue specimen storage

Answer 4

6 months

Question 5

iron staining pigment detected

Answer 5

iron pigment

Question 6

basic dye recommended for observing cell granules and vacuoles of phagocytic cells

Answer 6

hematoxylin

Question 7

commonly used dehydrating agent

Answer 7

alcohol and acetone

Question 8

refer to what during spills

Answer 8

material safety data sheet

Question 9

preservation of body fluids

Answer 9

refrigeratorw

Question 10

what day do we perform ihc staining

Answer 10

tuesday and friday

Question 11

what is main sol used for decalcifying bone/calcified tissue

Answer 11

decalcifying solution

Question 12

2 tissue adhesive

Answer 12

mayer’s egg albumin and poly-l-lysine

Question 13

tissue adhesive used in chh

Answer 13

poly-l-lysine

Question 14

organism detected using gms

Answer 14

fungi

Question 15

common faults in tissue sectioning

Answer 15

dull knife, knife edge is dirty, paraffin block is warm and soft

Question 16

2 types of fixative according to composition

Answer 16

simple and compound fixative

Question 17

what type of synthetic dye is eosin

Answer 17

acidic

Question 18

commonly used kind of eosin

Answer 18

eosin y

Question 19

advantages of using 10% nbf

Answer 19

cheap, easy to use, fast tissue penetration

Question 20

hematoxylin origin

Answer 20

logwood tree haematoxylum campechianum

Question 21

true or false
eosin stains cytoplasm yellow

Answer 21

false

Question 22

t or f
increase in temp slows the rate of penetration

Answer 22

false

Question 23

t or f
methylene blue is used as a color indicator for decalcifying agent

Answer 23

false

Question 24

t or f
carbol fuchsin is a counter stain that stains magenta red

Answer 24

false

Question 25

t or f
excess xylene favors bubbles on the slides

Answer 25

true

Question 26

t or f
wrights stain is used for bm staining

Answer 26

true

Question 27

t or f
her2dish is done every thursday

Answer 27

false

Question 28

t or f
50 degree c is the set temp for water bath

Answer 28

false

Question 29

t or f
higher conc of stain, the more ionization

Answer 29

true

Question 30

t or f
blotting should be done to dry up

Answer 30

false

Question 31

reagents/ solutions in decalcifying agent

Answer 31

50 gms trisodium citrate
90% formic acid
metanil yellow