Histology Stains

Question 1

basic dye carries what charge

Answer 1

positive

Question 2

basic dye binds what charge

Answer 2

negative

Question 3

basophilic molecules bind to

Answer 3

basic dyes

Question 4

three examples of basic dyes

Answer 4

methylene blue
toludine blue
hematoxylin

Question 5

nucleic acids

Answer 5

basophilic

Question 6

nuclei

Answer 6

basophilic

Question 7

nucleoli

Answer 7

basophilic

Question 8

ribosomes

Answer 8

basophilic

Question 9

glycoaminoglycans

Answer 9

basophilic

Question 10

acidic dyes carry what charge

Answer 10

negative

Question 11

acidic dyes bind to what charge

Answer 11

positive

Question 12

molecules that acidic dyes bind to are

Answer 12

eosinophilic or acidophilic

Question 13

5 examples of acidic dyes

Answer 13

eosin 
aniline blue
orange G
picric acid 
acid fuchsin

Question 14

collagen

Answer 14

eosinophilic

Question 15

keratin

Answer 15

eosinophilic

Question 16

hemoglobin

Answer 16

eosinophilic

Question 17

muscle proteins

Answer 17

eosinophilic

Question 18

describe metachromasia

Answer 18

shift in normal color that a dye would be (from blue to purple) in presence of polyanionic substances - super high charge - these molecules polymerize the dye molecules

Question 19

examples of toluidine blue metachromasia

Answer 19

mast cell granules (heparin and sulfated proteoglycans)

cartilage extracellular matrix (chondroitin sulfate proteoglycans)

Question 20

what is a Romanovsky type stain

Answer 20

a mix of acidic and basic dyes

Question 21

examples of Romanovsky stains

Answer 21

Wright Stain, Giemsa, Diffquick

Question 22

what are romanovsky stains typically used for

Answer 22

blood smears and cytology

Question 23

what is Periodic Acid Shift

Answer 23

used to identify CARBS
adjacent glycol linkages
shows: mucus, glycogen, glycocalyx, polysaccarides in basement membranes, fungal cell walls

Question 24

what is Silver Impregnation

Answer 24

looks for reticular fibers and nerve fibers
target stains black
fungi and spirochetes, type III collagen
neurofibrils, basement membrane, golgi

Question 25

what is Elastic Fiber (types and what is does)

Answer 25

aldehyde fuchsin, Verhoeff- Van Gieson, Orcein, Weigerts elastin elastic fibers- stain black

Question 26

what is Trichrome Stain

Answer 26

connective tissue smooth muscle form extracellular collagen collagen from nerve components (which are usually hard to distinguish with an H&E stain) collagen will appear blue green and cytoplasm red/orange to red/lilac

Question 27

Acid Fast Stain

Answer 27

organisms with host cell reactions | background will be green with magenta bacteria

Question 28

a mitochondria with H&E will appear

Answer 28

grainy eosinophilic

Question 29

an rER with Romanovsky

Answer 29

blue tinge

Question 30

Golgi with H&E or Romanovsky

Answer 30

appears as a pale empty zone

Question 31

histones with H&E

Answer 31

blue

Question 32

melanin with H&E, with Wright/Romanovsky

Answer 32

brown / dark-green-gray

Question 33

hemosiderin with H&E , with Prussian Blue

Answer 33

yellow/brown, blue/green

Question 34

Prussian Blue stains for

Answer 34

Fe, turns blue