Histology stains Flashcards
How to stain superficial dermatophyte?
- PAS
How to stain intracellular mucin?
- Mucicarmine (Carmine is inside)
- remember Hale’s colloidal iron stains extracellular mucin
How to stain extracellular mucin?
- Hale’s Colloidal iron
- Hale is outside
- (remember mucicarcime stains intracellular)
How to stain Amyloid?
- Congo red
How to stain Elastic fibers?
- EVG (Verhoeff von Gieson)
- gets Elastic fibers Very Good
How to stain atypical mycobacterium (e.g. in leprosy)
- Fite stain
How to stain melanin
- Fontana-Masson (For-Melanin)
How to stain deep fungal infections?
- GMS (Gomoris methenamine silver)
How to stain Hemosiderin?
- Perl’s prussian blue
How to stain mast cell granules?
- Giemsa
- toludine blue
- Leder stain
- mast cells are all about Gym tanning and laundry (GTL)
How to stain for Leishmaniasis?
- Giemsa
How to stain type I, II, and III minocycline pigmentation?
I= Perl's prussian blue II= Perls prussian blue and fontana masson III= Fontana masson
How to stain typical mycobacterium?
- Ziehl-Neelsen stain (AFB)
How to stain smooth muscle?
- Trichrome stain (will be red)
- Actin
- Desmin
How to stain fat?
- Scarlet red
- Sudan black
- osmium tetroxide
How to stain Calcium?
- Von Kossa (Kossa, Calcium)
How to stain Collagen?
- Trichrome
How to stain Glycogen?
- PAS with and without diastase
Stains that will differentiate dermatofibroma from DFSP?
- DFSP will be CD34 positive, this will be negative in DF
- DF will be CD 163 positive (negative in DSFP)
What stain for AXF?
CD10
What is a common endothelial cell marker on histopath?
ERG
