Histology stains Flashcards

1
Q

How to stain superficial dermatophyte?

A
  • PAS
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2
Q

How to stain intracellular mucin?

A
  • Mucicarmine (Carmine is inside)

- remember Hale’s colloidal iron stains extracellular mucin

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3
Q

How to stain extracellular mucin?

A
  • Hale’s Colloidal iron
  • Hale is outside
  • (remember mucicarcime stains intracellular)
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4
Q

How to stain Amyloid?

A
  • Congo red
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5
Q

How to stain Elastic fibers?

A
  • EVG (Verhoeff von Gieson)

- gets Elastic fibers Very Good

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6
Q

How to stain atypical mycobacterium (e.g. in leprosy)

A
  • Fite stain
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7
Q

How to stain melanin

A
  • Fontana-Masson (For-Melanin)
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8
Q

How to stain deep fungal infections?

A
  • GMS (Gomoris methenamine silver)
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9
Q

How to stain Hemosiderin?

A
  • Perl’s prussian blue
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10
Q

How to stain mast cell granules?

A
  • Giemsa
  • toludine blue
  • Leder stain
  • mast cells are all about Gym tanning and laundry (GTL)
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11
Q

How to stain for Leishmaniasis?

A
  • Giemsa
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12
Q

How to stain type I, II, and III minocycline pigmentation?

A
I= Perl's prussian blue
II= Perls prussian blue and fontana masson
III= Fontana masson
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13
Q

How to stain typical mycobacterium?

A
  • Ziehl-Neelsen stain (AFB)
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14
Q

How to stain smooth muscle?

A
  • Trichrome stain (will be red)
  • Actin
  • Desmin
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15
Q

How to stain fat?

A
  • Scarlet red
  • Sudan black
  • osmium tetroxide
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16
Q

How to stain Calcium?

A
  • Von Kossa (Kossa, Calcium)
17
Q

How to stain Collagen?

A
  • Trichrome
18
Q

How to stain Glycogen?

A
  • PAS with and without diastase
19
Q

Stains that will differentiate dermatofibroma from DFSP?

A
  • DFSP will be CD34 positive, this will be negative in DF

- DF will be CD 163 positive (negative in DSFP)

20
Q

What stain for AXF?

A

CD10

21
Q

What is a common endothelial cell marker on histopath?

A

ERG