Histology and Methods of Study

Question 1

What are the four types of Tissues?

Answer 1

1. Epithelial Tissues
2. Nervous Tissues
3. Muscle Tissues
4. Connective Tissues

Question 2

What are epithelial tissues classified by?

Answer 2

Shape and Layers

Question 3

What is the function of epithelial tissues?

Answer 3

Protection, Secretion, Absorption and focus barriers. It also covers organs and forms glands.

Question 4

Describe the features of epithelial tissues

Answer 4

Close cell contact
Avascular
Innervated
Regenerative
Polar Cell

Question 5

Name four Stainings

Answer 5

1. Hematoxyline + Eosin (H&E staining )
2. Periodic acid - Schiff
3. Lipid Soluble dyes

Question 6

What is metal impregnation?

Answer 6

A technique that allows for the mapping of brain regions by visualisation of neuronal connections

Question 7

What is Autoradiography?

Answer 7

A method of localising newly synthesised macromolecules in cells or tissue sections

Question 8

What is Enzyme Histochemistry?

Answer 8

A method for localising cellular structures using specific enzymatic activity present in those structures

Question 9

What are examples of molecules that interact specifically with other molecules? (Immunohistochemistry)

Answer 9

1. Phalloidin - interacts strongly with the actin protein of microfilaments
2. Protein A - binds to Fc region of antibody molecules
3. Lectins - Bind to carbohydrates with high affinity and specificity.

Question 10

Describe the hybridisation technique

Answer 10

1. Determine if a cell has a specific sequence of DNA, such as a gene
2. Identify the cells containing specific messenger RNAs to which the corresponding gene is being transcribed
   3.Determinating the localisation of a gene in a specific chromosome

Question 11

Describe the method of Autoradiography

Answer 12

1. Radiolabled cells are coated in photographic emu