Histological Techniques Flashcards
1
Q
Define resolution
A
the ability to distinguish two adjacent bodies as being separate
2
Q
What is resolution limited by?
A
the wavelength of the illuminating beam
3
Q
What stains are used for transmission electron microscopy (TEM)?
A
Heavy Metal Ions
4
Q
What does it mean to “fix” a tissue sample?
A
Treat it with a chemical or physical agent that preserves its native structure, as much as possible
5
Q
What is a common fixative for LM?
A
Formaldehyde
6
Q
What is a common fixative for EM?
A
Glutaraldehyde
7
Q
When does fluorescence occur?
A
When a molecule absorbs light at one wavelength, and then emits it at a longer wavelength
8
Q
What is an antigen?
A
any material that, when injected into an animal, elicits the production of antibodies
