High Yield Protein Trafficking

Question 1

Acetylation occurs at what AA(s)?

Answer 1

Lysine

Question 2

Glycosylation occurs at what AA(s)?

Answer 2

Serine, threonine, asparagine, and glutamine

Question 3

Phosphorylation occurs at what AA(s)?

Answer 3

Serine, tyrosine, threonine; sometimes aspartic acid and histidine

Question 4

Disulfide bonds occur at what AA(s)?

Answer 4

Cysteines

Question 5

What translocation signal would you find on proteins destined for the cytoplasm?

Answer 5

None

Question 6

What translocation signal would you find on proteins destined for the mitochondria?

Answer 6

Hydrophobic alpha-helix

Question 7

What translocation signal would you find on proteins destined for the nucleus?

Answer 7

Lysine and arginine rich

Question 8

What translocation signal would you find on proteins destined for the peroxisome?

Answer 8

Serine, lysine, leucine

Question 9

What translocation signal would you find on proteins destined for the ER lumen?

Answer 9

Lysine, aspartic acid, glutamic acid, and leucine (KDEL)

Question 10

What translocation signal would you find on proteins destined for the lysosome?

Answer 10

M6P

Question 11

What translocation signal would you find on proteins destined for secretion?

Answer 11

Tryptophan rich

Question 12

What translocation signal would you find on proteins destined for the plasma membrane?

Answer 12

Apolar region (stop-transfer sequence)

Question 13

What signal do all proteins in the secretory pathway share at the beginning of the peptide?

Answer 13

N-terminal hydrophobic alpha-helix ER signal peptide