HIC chromatography Procedure Flashcards
1
Q
HIC chromatography procedure (TLICC)
A
TE buffer, Lysosome, Ice (dry), centrifuge, column
2
Q
TE buffer purpose
A
suspend the bacteria in the TE buffer
3
Q
purpose of adding lysozyme
A
lyses open the cells
4
Q
purpose of incubating on dry ice
A
further lyse open cells
5
Q
purpose of centrifuging sample
A
GFP is in the supernatant
6
Q
purpose of running supernatant through HIC column
A
separate GFP from the rest of the junk
7
Q
steps of running supernatant through HIC column (ESWE)
A
Add Equilibration Buffer (Set ideal conditions for GFP)
Add solution (supernatant from step 4)
Add Wash Buffer (get rid of all the junk)
Add Elution Buffer (elute the GFP)
