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Methoden > HF1 > Flashcards

HF1 Flashcards

1
Q

lyseren voor verkrijgen van DNA

A

humaan klein weefsel
grote weefsels
boed
bacteriën

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2
Q

fenol-chloroform

A

isoleren van DNA

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3
Q

cesium-chloride densiteit chromatografie

A

isoleren van DNA

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4
Q

alcohol precipitate

A

concentreren van DNA

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5
Q

concentrator en Speedvac

A

concentreren van DNA

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6
Q

dipstick

A

kwantificatie DNA

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7
Q

OD260

A

kwantificatie DNA

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8
Q

enzymatische assay

A

kwantificatie DNA

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9
Q

kwantificatie DNA

A

OD, dipstick, enzymatische assay, gelkwantificering

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10
Q

manipuleren en kloneren van DNA

A

restrictie digestie, aspecifieke fragmentatie, uiteinde invullen, uiteinden afknabbelen, merken van DNA, scheiding op gel, vector, ligeren, TA-cloneren, kloneren met recombinatie

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11
Q

primaire sequentie bepaling

A

chemische en Sanger

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12
Q

NGS

A

454/Roche, Illumina, nanopore, ion/proton sequencing, PacBio (ABI-SOLID)

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13
Q

Southernblot

A

genidentificatie DNA

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14
Q

RFLP

A

genidentificatie DNA

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15
Q

ARMS

A

genidentificatie DNA

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16
Q

melting analysis

A

genidentificatie DNA

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17
Q

comparatieve genomic hybridization

A

genidentificatie DNA

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18
Q

oligonucliotiden assay

A

genidentificatie DNA

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19
Q

Q-RT-PCR

A

genidentificatie DNA

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20
Q

genidentificatie DNA

A

southernblot, RFLP, ARMS, melting analysis, cooperative genomes, oligonucl assay, Q-RT-PCR

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21
Q

quanidine isothiocyanaat/fenol

A

RNA bereiding

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22
Q

ionenuitwisselingschromatigrafie

A

isoleren van DNA + Bereiding RNA

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23
Q

RNA bereiding

A

quanidine isothiocyanaat/fenol en ionenuitwisselingschromatigrafie

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24
Q

northernblot

A

transcriptieidentificatie RNA

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25
Q

RT-PCR

A

transcriptieidentificatie RNA

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26
Q

transcriptie identificatie

A

northern blot, RT-PCR, in situ hyridisatie

27
Q

Detectie in PCR

A

Taqman, SYBRGreen, Molecular beacon, FRET

28
Q

DNA microarray

A

nylon, glas, fotolythografisch

+kleur manieren!!

29
Q

single cell transcription

A

DROP-seq

CITE-seq

30
Q

DROP-seq

A

single cell transcription RNA

31
Q

CITE-seq

A

single cell transcription RNA

32
Q

spatial transcriptoom

A

geo-seq, in situ seq

33
Q

aanmaak van eiwitten

A

chemisch (f-moc), enzymatisch (wheat germ), in vivo, Ab

34
Q

uitzouten

A

scheiden van eiwitten

35
Q

gel-elektroforese op eiwitten

A

scheiden van eiwitten

36
Q

chromatografie op eiwitten

A

scheiden van eiwitten

37
Q

scheiden van eiwitten

A

uitzouten, gel-elektroforese, chromatografie

38
Q

concentratiebepaling van eiwitten

A

UV, colorimetrisch, fluometrisch

39
Q

colorimetrische testen

A

concentratie bepaling eiwitten

40
Q

eiwitidentificatie

A

kleuren in gel, ELISA, RIA, Edman, westernblot, MS/MS, MALDI, immunohistochemie, delfia

41
Q

ELISA

A

eiwitidentificatie

42
Q

RIA

A

eiwitidentificatie

43
Q

Delfia

A

eiwitidentificatie

44
Q

westernblot

A

eiwitidentificatie

45
Q

Edman

A

eiwitidentificatie

46
Q

exploratieve proteomics

A

bottom-up: 2D-gel, chromatografie

47
Q

targetted proteomics

A

MS-based, SOMAmer

48
Q

differentiele proteomics

A

2D-DIGE, SILAC, ICAT, ITRAQ

49
Q

NGP

A

portie fingerprint fluo, nanopore, e-tunneling

50
Q

spatial proteomics

A

MILAN, IMC, MS-imaging

51
Q

single cell eiwit analyse

A

single cell westerns, CyTOF

52
Q

CyTOF

A

single cell eiwit analyse

53
Q

eiwit-eiwit interactie

A

yeast-2-hybrid, MAPPIT, affinity pull down, FRET, RIME, proximity ligation test, array based, immunoprecipitatie, SPR, faag

54
Q

yeast-2-hybrid

A

eiwit-eiwit interactie

55
Q

MAPPIT

A

eiwit-eiwit interactie

56
Q

affinity pull down

A

eiwit-eiwit interactie

57
Q

RIME

A

eiwit-eiwit interactie

58
Q

SPR

A

eiwit-eiwit interactie

59
Q

proximity ligation test

A

eiwit-eiwit interactie

60
Q

analyse gentranscriptie

A

run-on, promotor-reporter, DNase foot printing,DNAse seq, FAIRE, EMSA, affinity chromatography, chip

61
Q

chip

A

analyse gentranscriptie

62
Q

FAIRE

A

analyse gentranscriptie

63
Q

EMSA

A

analyse gentranscriptie

64
Q

DNAse footprinting

A

analyse gentranscriptie

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