Hemolytic Disease Of the Newborn/Autoimmune Disease/Alternative procedures

Question 1

Eryhroblastosis fatalis

Answer 1

Hemolytic Disease of the Newborn

Question 2

Fetal red cells are coated with maternal antibodies that correspond to specific fetal antigen

Answer 2

Hemolytic Disease of the Newborn

Question 3

Caused by immune _____ from the mother crossing the placenta and agglutinating fetal cells

Answer 3

Isoagglutinins

Question 4

Condition for HDN to occur:

Answer 4

Baby should be positive for the antigen
Mother should be negative for antigen
Mother must contain specific Abs
Ab must be IgG

Question 5

Unconjugated bilirubin causing kernicterus

Answer 5

> 18g/dL

Question 6

Most common type of HDFN

Answer 6

ABO HDN causing mild jaundice

Question 7

Most severe type of HDFN
Presence of Stomatocytosis

Answer 7

Rh HDN

Question 8

HDN can be caused by:

Answer 8

Duffy, MNS, Kidd,Kell

Question 9

HDN cannot be caused by:

Answer 9

Lewis, I,P1

Question 10

Amniotic fluid absorbance is determined using ______

Answer 10

Spectrophotometric assay

Question 11

At 365-550nm; bilirubin peaks at _____

Answer 11

450nm

Question 12

Plot of absorbance in _______ (Absorbance against gestational age)

Answer 12

Liley graph

Question 13

Mildly affected with HDN

Answer 13

Zone 1

Question 14

Moderately affected (careful monitor)

Answer 14

Zone 2

Question 15

Severe in Liley graph

Answer 15

Zone 3

Question 16

Rh antibody production is stimulated during _______

Answer 16

delivery, wherein fetomaternal hemorrhage occurs

Question 17

at least _____ of fetal RBC pass into maternal circulation

Answer 17

30mL

Question 18

to prevent HDFN, Rh (-) mothers most be given ______

Answer 18

Rhogam

Question 19

at ___ weeks gestation, the mother must be given Rhogam or within ____ hours after delivery of Rh D

Answer 19

28 weeks, 72 hours

Question 20

Qualitative determination for Fetomaternal hemorrhage

Answer 20

Fetal screen Rossette method

Question 21

Quantitative determination for fetomaternal hemorrhage

Answer 21

Kleihauer Betke Acid Elution Test

Question 22

Uses citric acid buffer and causes maternal cells to burst while fetal cells remain intact

Answer 22

Kleihauer Betke Acid Elution Test

Question 23

FMH formula

Answer 23

FMH=(% fetal cells x maternal blood volume)/100

Question 24

Average maternal blood volume

Answer 24

5000mL

Question 25

Most common type of autoimmune hemolytic anemia (Ab have rh-like specificity)

Answer 25

WAIHA

Question 26

Antibodies are reactive against colder temperature

Answer 26

CAIHA

Question 27

Result of a patient’s antibody production in response to a specific drug or drug complex

Answer 27

DAIHA

Question 28

Developed by Yves Lappiere

Answer 28

Gel technology

Question 29

Controlled centrifugation of RBC in Gel technology is done through a ___

Answer 29

Dextran polyacrylamide

Question 30

Gel card dimension:

Answer 30

5x7cm with 6 microtubes

Question 31

Major advantage of gel technology

Answer 31

Standardization

Question 32

Disadvantage of Gel technology

Answer 32

Sample restriction and need for special equipment

Question 33

Solid band of agglutinated RBCs at the TOP of the column

Answer 33

4+

Question 34

Majority of agglutinates are in the TOP of the column

Answer 34

3+

Question 35

Agglutinates distributed throughout upper and lower halves of column

Answer 35

2+

Question 36

Majority of agglutinates are at the lower half of the column

Answer 36

1+

Question 37

Rbcs form well delineated pellet

Answer 37

Negative

Question 38

Layer of agglutinates at the top of the gel and a pellet of delineated cells at the bottom

Answer 38

Mixed field

Question 39

Positive reaction for solid phase technology

Answer 39

Red cells will form a monolayer when indicator cells are added

Question 40

Negative reaction for solid phase technology

Answer 40

The indicator cells form a pellet at the bottom of the well