Hemolymphatic Flashcards
Polycythemia vera (primary erythrocytosis) - treatment options?
Rpx phlebotomy
Chemo agents (e.g. hydroxyurea)
Hirudotherapy (medicinal use of
leeches)
Onion powder (induce hemolysis) - case report in a cat
Polycythemia vera (primary erythrocytosis) - CSx and pathogenesis?
Cats: CSx common - congested MM, neuro signs. +/- GI signs.
Patho - as in humans, gain-of-function mutation in exon 12 of the JAK2 gene
leading to erythrocytosis only. But true underlying cause unknown.
Define leukoreduction.
Methods of preserving blood to optimize its characteristics and limit degradation during storage are required. In particular, the metabolites of leukocytes such as cytokines, histamine, elastase, and acid phosphatase
seem fundamental to the development of storage lesions and post-transfusion reactions.
In humans, the leukoreduction of the human blood units reduces number of WBCs (about 1-5 x 10^6 per blood unit). The prestorage filtration, soon after the blood donation,
reduces the lesions of RBCs during the storage period by removing the leukocytes before their fragmentation and avoiding the accumulation of cytokines of leukocyte origin in stored blood and blood components.
1) Why are RBCs susceptible to oxidative injury? Esp cats?
2) What mechanisms do RBCs have to prevent oxidative injury?
3) Clin path findings with RBC oxidative injury?
1) Risk factors for oxidative injury: ubiquity, proximity to oxygen, lack of
nuclear material, high iron content.
Cats - feline Hb contains 8 instead of 2 oxidizable sulfhydryl groups. Also feline spleen is inefficient at removing HB. So low numbers (<10%) single, small Heinz bodies may be seen on RBCs in healthy cats.
2) Most impt mechanism = glutathione pathway. Glutathione is a tripeptide produced from cysteine,
glycine & glutamate, and is primarily formed and stored by the liver,
but RBCs have intracellular glutathione as a major antioxidant
defense. Glutathione exerts its antioxidant effect through neutralizing ROS. Glutathione, in the presence of ROS is oxidized and the interaction of free radicals and enzymes like glutathione peroxidase form oxidized glutathione (GSSG). Oxidized glutathione can be recycled through the function of enzymes (eg, glutathione reductase) and cofactors (eg, vitamin C, vitamin E, and selenium), to be reduced to its original form (reduced glutathione, GSH).
3) Heinz bodies, eccentrocytes
Heinz bodies
a) Formation process?
b) DDx in cats?
c) DDx in dogs?
d) Which stain to differentiate?
a) HBs are produced when the sulfhydryl groups in the globin part of the Hb molecule undergoes oxidation, causing the molecule to become unstable –> damaged Hb molecules coalesce. Usually removed from RBCs by the spleen.
b) DKA > DM, hepatic lipidosis, drugs (acetaminophen), hyperT (with/without anemia), lymphoma, renal failure, propylene glycol (semi-moist food - can see up to 50% HBl; usually not anemic but reduced RBC lifespan), salmon based diets.
c) Onions/garlic, Zn, paracetamol (< cats)
d) New methylene blue (HB stain dark blue, RBC pale aqua)
Cat RBCs. Abnormality?
Heinz bodies
Lifespan of RBCs in dogs vs cats?
D: 110-120 days
C: 65-76 days
Name 2 congenital platelet disorders in dogs.
1) Platelet storage pool disease (impaired adenosine diphosphate (ADP) storage in the dense granules of platelets)
2) Glanzmann’s thrombasthenia (impaired expression of platelet integrin αIIbβ3)
What is the pathogenesis of Glanzmann’s thrombasthenia?
Autosomal recessive congenital platelet disorder caused by qualitative or quantitative defects of platelet GPIIb–IIIa complex (aka fibrinogen receptor), due to mutations within the gene encoding GPIIb.
Leads to impaired platelet aggregation, platelets cannot bind fibrinogen and mediate clot retraction.
Clinical manifestations of Glanzmann’s thrombasthenia & findings on platelet function testing?
Bleeding diathesis; intermittent hematoma formation and/or petechiae hemorrhage, onset of signs from young.
Whole blood platelet aggregometry - significant impairment in platelet aggregation in response to ADP and AA.
Dog & cat breeds affected by Glanzmann’s thrombasthenia?
All cases reported involve mutations in gene encoding GPIIb.
Dogs:
- Otterhounds - single nucleotide change in exon 12 –> substitution of a histidine for aspartic acid within the 3rd calcium-binding domain of GPIIb.
- Great Pyrenees (Type 1 GT - severe quantitative decrease in GPIIb–IIIa). 14-base-pair repeat in exon 13 –> frameshift –> premature stop codon
- Mixed breed dogs (case report Haysom JVIM 2016) - SNP in exon 13 –> premature stop codon at codon for arginine.
Cat: case report (non-pedigree) Li JVIM 2020.
ITGA2B gene –> frameshift –> impaired expression of platelet integrin αIIbβ3.
Incidence of transfusion reactions in dogs?
Most common type of transfusion reaction?
3-28%.
Febrile non-hemolytic reaction.
Describe major vs minor cross-matching.
Major CM – detects recipient antibodies against donor RBC antigens.
Minor CM test – detects antibodies in the donor serum against recipient RBC antigens.
Presence of agglutination or haemolysis indicates incompatibility.
Role of protein C?
Consequences of protein C deficiency?
Circulating, vitamin K-dependent protease produced by the liver. Activated form has a role in regulating coagulation - acts as an anticoagulant by inactivating factors Va & VIIIa –> regulates subsequent thrombin generation.
Venous thrombosis (case report in dog)
Which other novel erythrocyte antigens have been identified in cats outside of the AB system? What is the prevalence of these antigens (if known)?
What is the clinical significance of knowing antigen status in cats receiving blood transfusions?
Binvel JVIM 2020
Mik
FEA 1 - 84%
FEA 5 - 96%
And 3 other FEAs (2-4)
FEA 1-negative status was associated with a higher risk of having naturally occurring alloantibodies (NOAb) - present in 16.7% cats (vs 5.1% of FEA 1-positive cats).
FEA 1 may correspond to the Mik antigen. Some FEA 1 or Mik-negative cats may present anti-Mik or anti-FEA 1 NOAb - may mediate a clinically relevant transfusion reaction despite blood donor and recipient being AB-matched.