Hemoglobin & Myoglobin Flashcards

1
Q

_____ is a complex of protoporphyrin IX and ferrous ion (Fe2+)

A

Heme

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2
Q

What does heme do in hemoglobin and myoglobin?

A

it serves to reversibly bind oxygen

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3
Q

Where is Myoglobin present?

A

Heart and skeletal muscle

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4
Q

What is the function of myoglobin?

A

reservoir of oxygen in red muscle fibers

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5
Q

When is the oxygen that is stored in myoglobin released?

A

During oxygen deprivation

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6
Q

Does myoglobin have a high or low molecular weight?

A

Low ~17,000

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7
Q

Where is Hemoglobin present?

A

in RBCs

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8
Q

What is the function of Hemoglobin?

A

it reversibly carries oxygen from lungs to peripheral tissues, and H+ and some CO2 from peripheral tissues to the lungs.

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9
Q

HbA has what?

A

2 alpha, 2 beta

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10
Q

HbF has what?

A

2 alpha, 2 gamma

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11
Q

HbA2 has what?

A

2 alpha, 2 delta

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12
Q

How long does embryonic hemoglobin persist?

A

from 15 days after fertilization until 6 weeks of embryonic life

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13
Q

When does HbF predominate?

A

from 6 weeks of fetal life; gradually falls from 30 weeks of prenatal life. At birth and up until 4-5 weeks of post natal life, HbF is the predominate type.

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14
Q

When completely saturated, how many oxygens do myoglobin and hemoglobin bind?

A

myoglobin binds one O2 and hemoglobin binds 4

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15
Q

In the absence of oxygen (T form), the interaction between (αβ)1- (αβ)2 is ______

A

tight

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16
Q

In the presence of oxygen (R form), the interaction between (αβ)1- (αβ)2 is ______

A

less tight

17
Q

______ is the degree of saturation measured at different partial pressures of oxygen

A

Oxygen dissociation curve

18
Q

2,3-BPG ______ the oxygen affinity of hemoglobin

A

decreases

19
Q

What is 2,3-BPG’s effect on deoxyhemoglobin and hemoglobin?

A

it stabilizes deoxyhemoglobin and helps to release O2 from hemoglobin.

20
Q

The levels of 2,3-BPG _____ in response to chronic hypoxia and helps to release O2 from hemoglobin

A

increase

21
Q

Factors that cause a right shift (reduced affinity) to the oxygen-hemoglobin dissociation curve

A
  • increase in 2,3-BPG
  • increase in PCO2
  • decrease in pH
22
Q

Factors that cause a left shift (increased affinity) to the oxygen-hemoglobin dissociation curve

A
  • decreased 2,3-BPG
  • decrease in PCO2
  • increased pH
  • presence of CO
23
Q

____ glucose levels in uncontrolled type 2 DM will increase HbA1c

A

Higher

24
Q

What is the most abundant form of glycosylated hemoglobin?

A

HbA1c

25
Q

What is used to monitor the long term control of blood glucose in DM?

A

HbA1c

26
Q

What causes the release of O2 from hemoglobin?

A

low tissue pH and high PCO2

27
Q

How does CO bind to the iron of hemoglobin?

A

covalently, but reversibly

28
Q

Where is embryonic hemoglobin produced?

A

in the yolk sac

29
Q

Where are the major sites of fetal erythropoiesis?

A

liver and some spleen

30
Q

In the last few weeks before birth, what is the site of erythrocyte formation?

A

bone marrow

31
Q

Which histidine, proximal or distal, directly interacts with Fe2+ of heme?

A

His F8(proximal)

32
Q

Which histidine, proximal or distal, does not directly interact with Fe2+ of heme, but helps to stabilize the binding of oxygen to the ferrous ion?

A

His E7(distal)

33
Q

10% of total CO2 produced in peripheral tissue is carried in the form of what?

A

carbaminohemoglobin

34
Q

90% of total CO2 produced in peripheral tissue is carried in the form of what?

A

bicarbonate